2-Methylcitrate cycle: a well-regulated controller of Bacillus sporulation
2-Methylcitrate cycle: a well-regulated controller of Bacillus sporulation
复制标题
2-甲基柠檬酸循环：芽孢杆菌孢子形成的良好调节控制器
DOI：
10.1111/1462-2920.14901
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发表时间：
2020
影响因子：
5.1
通讯作者：
He Jin
中科院分区：
文献类型：
--
作者：
Zheng Cao;Yu Zhaoqing;Du Cuiying;Gong Yujing;Yin Wen;Li Xinfeng;Li Zhou;Roemling Ute;Chou Shan-Ho;He Jin
苏云金芽孢杆菌是使用最广泛的环保生物农药，含有生物防治的两个主要决定因素：内生孢子和杀虫晶体蛋白（ICP）。 2-甲基柠檬酸循环是一种广泛存在的碳代谢途径，在丙酰辅酶A的通道中发挥着至关重要的作用，但其代谢调节机制知之甚少。在这里，我们剖析了2-甲基柠檬酸循环操纵子prpCDB的转录调控，并报告了其在控制B.孢子形成过程中前所未有的作用。苏云金。我们发现编码2-甲基柠檬酸循环中三种关键酶PrpC、PrpD和PrpB的prpoperon的转录活性受到两个全局转录因子CcpA和AbrB的负向调节，而受到LysR家族调节因子CcpC的正向调节，这共同解释了2-甲基柠檬酸循环在静止状态下具有特异性和高度活性的事实。 成长阶段。我们还发现prpD突变体积累了2-甲基柠檬酸，这是2-甲基柠檬酸循环的中间代谢产物，在早期阶段延迟并抑制了孢子形成。因此，我们的结果不仅揭示了代谢 2-甲基柠檬酸循环的复杂转录调节机制，而且还确定了 2-甲基柠檬酸作为 B 孢子形成的新型调节剂。苏云金。
Bacillus thuringiensisis the most widely used eco‐friendly biopesticide, containing two primary determinants of biocontrol, endospore and insecticidal crystal proteins (ICPs). The 2‐methylcitrate cycle is a widespread carbon metabolic pathway playing a crucial role in channelling propionyl‐CoA, but with poorly understood metabolic regulatory mechanisms. Here, we dissect the transcriptional regulation of the 2‐methylcitrate cycle operonprpCDBand report its unprecedented role in controlling the sporulation process ofB. thuringiensis. We found that the transcriptional activity of theprpoperon encoding the three critical enzymes PrpC, PrpD, and PrpB in the 2‐methylcitrate cycle was negatively regulated by the two global transcription factors CcpA and AbrB, while positively regulated by the LysR family regulator CcpC, which jointly account for the fact that the 2‐methylcitrate cycle is specifically and highly active in the stationary phase of growth. We also found that theprpDmutant accumulated 2‐methylcitrate, the intermediate metabolite of the 2‐methylcitrate cycle, which delayed and inhibited sporulation at the early stage. Thus, our results not only revealed sophisticated transcriptional regulatory mechanisms for the metabolic 2‐methylcitrate cycle but also identified 2‐methylcitrate as a novel regulator of sporulation inB. thuringiensis.