EXCESS INFORMATION AT BACTERIOPHAGE-T7 GENOMIC PROMOTERS DETECTED BY A RANDOM CLONING TECHNIQUE
EXCESS INFORMATION AT BACTERIOPHAGE-T7 GENOMIC PROMOTERS DETECTED BY A RANDOM CLONING TECHNIQUE
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DOI：
10.1093/nar/17.2.659
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发表时间：
1989-01-25
影响因子：
14.9
通讯作者：
STORMO, GD
中科院分区：
文献类型：
--
作者：
SCHNEIDER, TD;STORMO, GD
在我们之前对核酸结合位点信息的分析中，我们发现大多数检测的位点包含的信息量与它们在基因组中的频率相同。噬菌体T7启动子的序列是一个例外，因为它们比将它们与大肠杆菌基因组背景（17位）区分开来所需的保守得多（35位信息内容）。为了确定T7 RNA聚合酶实际使用的信息，化学合成具有许多变体的启动子，并对在体内测定中功能良好的启动子进行测序。我们的分析表明，聚合酶使用18位的信息，因此噬菌体基因组启动子的序列具有比聚合酶需要的更多的信息。过量可能代表另一种蛋白质的结合位点。
In our previous analysis of the information at binding sites on nucleic acids, we found that most of the sites examined contain the amount of information expected from their frequency in the genome. The sequences at bacteriophage T7 promoters are an exception, because they are far more conserved (35 bits of information content) than should be necessary to distinguish them from the background of the Escherichia coli genome (17 bits). To determine the information actually used by the T7 RNA polymerase, promoters were chemically synthesized with many variations and those that function well in an in vivo assay were sequenced. Our analysis shows that the polymerase uses 18 bits of information, so the sequences at phage gnomic promoters have significantly more information than the polymerase needs. The excess may represent the binding site of another protein.