Bacillus subtilis genome diversity
Bacillus subtilis genome diversity
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DOI：
10.1128/jb.01343-06
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发表时间：
2007-02-01
影响因子：
3.2
通讯作者：
Kolter, Roberto
中科院分区：
文献类型：
--
作者：
Earl, Ashlee M.;Losick, Richard;Kolter, Roberto
基于微阵列的比较基因组杂交（M-CGH）是一种快速鉴定近缘生物基因组多样性区域的有效方法。我们使用M-CGH检测了17株属于非致病性枯草芽孢杆菌的基因组多样性。我们的M-CGH结果表明，该物种成员之间存在相当大的遗传异质性；通过微阵列杂交强度测量，近三分之一的bsu168特异性基因表现出可变性。可变基因座包括那些编码参与抗生素生产、细胞壁合成、产孢和萌发的蛋白质的基因座。这些基因的多样性可能反映了这种生物在不同自然环境中生存的能力。
Microarray-based comparative genomic hybridization (M-CGH) is a powerful method for rapidly identifying regions of genome diversity among closely related organisms. We used M-CGH to examine the genome diversity of 17 strains belonging to the nonpathogenic species Bacillus subtilis. Our M-CGH results indicate that there is considerable genetic heterogeneity among members of this species; nearly one-third of Bsu168-specific genes exhibited variability, as measured by the microarray hybridization intensities. The variable loci include those encoding proteins involved in antibiotic production, cell wall synthesis, sporulation, and germination. The diversity in these genes may reflect this organism's ability to survive in diverse natural settings.