Neuropilin-2 is required for the proper targeting of ventral glomeruli in the mouse olfactory bulb
Neuropilin-2 is required for the proper targeting of ventral glomeruli in the mouse olfactory bulb
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DOI：
10.1016/j.mcn.2010.03.010
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发表时间：
2010-07-01
影响因子：
3.5
通讯作者：
Tsuboi, Akio
中科院分区：
文献类型：
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作者：
Takahashi, Hiroo;Yoshihara, Sei-ichi;Tsuboi, Akio
最近的证据表明，表达给定气味受体（OR）的嗅觉感觉神经元不一定局限于四个区域之一，而是在嗅觉上皮（OE）中以重叠方式排列。在这项研究中，OE切片与OR探针的原位杂交表明，OR基因（其mRNA在沿前背/后腹（AD/PV）轴的嗅球（OB）上的肾小球阵列中检测到）在最腹侧区域（区域4）内沿背内侧/腹外侧（DM/VL）轴的亚区域中表达。我们还发现 Neuropilin-2 (Nrp2) 在 OE 区域 4 内以 DM-低至 VL-高梯度表达。此外，在 Nrp2 突变小鼠中，我们观察到 OB 中 4 区 OR 的多个肾小球。这些结果表明，在 OE 中 Nrp2 的分级表达对于在 OB 中沿 AD/PV 轴正确定位腹侧肾小球是必需的。 (C) 2010 Elsevier Inc. 保留所有权利。
Recent evidence shows that olfactory sensory neurons expressing a given odorant receptor (OR) are not necessarily confined to one of four zones, rather arranged in an overlapping manner in the olfactory epithelium (OE). In this study, in situ hybridization of OE sections with the OR probes indicated that the OR genes, the mRNAs of which were detected in an array of glomeruli on olfactory bulb (OB) along the anterodorsal/posteroventral (AD/PV) axis, are expressed in subareal zones within the most ventral zone, zone 4, along the dorsomedial/ventrolateral (DM/VL) axis. We also found that Neuropilin-2 (Nrp2) is expressed in a DM-low to VL-high gradient within zone 4 of OE. Furthermore, in Nrp2 mutant mice, we observed multiple glomeruli for zone 4 ORs in OB. These results suggest that the graded expression of Nrp2 in OE is required for the proper targeting of ventral glomeruli along the AD/PV axis in OB. (C) 2010 Elsevier Inc. All rights reserved.