Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix.
Bioadhesive characterization of poly(methylidene malonate 2.12) microparticle on model extracellular matrix.
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模型细胞外基质上聚（亚甲基丙二酸酯 2.12）微粒的生物粘附特性。
DOI：
10.1016/j.biomaterials.2003.11.021
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Leong,KamW
中科院分区：
文献类型：
--
作者：
Chan,Vincent;Liu,Kuo-Kang;LeVisage,Catherine;Ju,Bin-Feng;Leong,KamW
药物递送系统的功效取决于其在靶组织中的保留。微粒是最流行和最有效的药物递送结构之一。最近，研究表明载药微粒与组织之间的相互作用与将紫杉醇递送至小鼠膀胱壁治疗浅表性膀胱癌的有效性有关。在本研究中，利用共焦反射干涉对比显微镜（C-RICM）、单颗粒压缩力测量和接触力学理论探讨了聚丙二酸亚甲基酯2.12或PMM 2.1.2微粒与作为膀胱壁细胞外基质模型的胶原蛋白之间的粘附相互作用。测定单个PMM 2.1.2微粒的杨氏模量为1.56±0.25×104N/m2。对于水中的普通 PMM 2.1.2 微粒（pH 5.5），随着中面直径从 2μm 增加到 18μm，胶原涂层基底上的变形度（a/R）从 0.77 降低到 0.26。根据Maguis-JKR理论确定PMM 2.1.2微粒的粘附能，随着粒径的增加，粘附能始终保持在1.5mJ/m2左右。在pH 4时，与pH 5.5相比，颗粒变形的平均程度和粘附能分别增加了11%和32%。 PMM 2.1.2 微球中紫杉醇的负载增强了微球在 pH 5.5 下的变形和粘附。假设pH 4 时紫杉醇和胶原蛋白之间的静电排斥降低了PMM 2.1.2-紫杉醇微球的粘附能。这项研究可以通过提供实验和理论工具来研究载药微粒与模型细胞外基质之间的生物粘附相互作用，从而为未来微粒递送系统的设计提供见解。
The efficacy of a drug delivery system is predicated on its retention in the target tissue. Microparticle is one of the most popular and effective drug delivery configurations. Recently, it has been shown that the interaction between drug-loaded microparticles and tissues is related to the effectiveness of paclitaxel delivery to the bladder wall of mice for treating superficial bladder cancer. In this study, the adhesive interaction between poly(methylidene malonate 2.12) or PMM 2.1.2 microparticles and collagen, which serves as the model extracellular matrix for bladder wall, was probed with confocal reflectance interference contrast microscopy (C-RICM), single-particle compressive force measurement and contact mechanics theory. Young's modulus of single PMM 2.1.2 microparticle was determined as 1.56±0.25×104N/m2. For plain PMM 2.1.2 microparticle in water (pH 5.5), the degree of deformation (a/R) on collagen coated substrate decreased from 0.77 to 0.26 against the increase of mid-plane diameter from 2 to 18μm. The adhesion energy of PMM 2.1.2 microparticle was determined from Maguis-JKR theory and remained at around 1.5mJ/m2against the increase of particle diameter. At pH 4, the average degree of particle deformation and adhesion energy was increased by 11% and 32%, respectively, in comparison with that at pH 5.5. The loading of paclitaxel in PMM 2.1.2 microspheres enhanced the deformation and adhesion of microspheres at pH 5.5. It is hypothesized that the electrostatic repulsion between paclitaxel and collagen at pH 4 reduces the adhesion energy of PMM 2.1.2-paclitaxel microsphere. This study may offer insight for design of future microparticulate delivery systems by providing the experimental and theoretical tools to study the bioadhesive interaction between drug-loaded microparticles and model extracellular matrices.