Evaluation of Retinal Circulation Using Segmental-Scanning Doppler Optical Coherence Tomography in Anesthetized Cats
Evaluation of Retinal Circulation Using Segmental-Scanning Doppler Optical Coherence Tomography in Anesthetized Cats
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DOI：
10.1167/iovs.15-18303
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发表时间：
2016-06-01
影响因子：
4.4
通讯作者：
Yoshida, Akitoshi
中科院分区：
文献类型：
--
作者：
Nagaoka, Taiji;Tani, Tomofumi;Yoshida, Akitoshi
目的.目的研究节段扫描多普勒光学相干断层扫描（DOCT）测量视网膜血流量（RBF）的重复性。作为初步研究，通过使用输液泵改变人血通过玻璃毛细管的流速，并在13个预设速度下用DOCT测量。对于体内测量，使用七氟烷麻醉猫。将载瘤血管中的流量与两个子血管中的流量值之和进行比较。RBF使用两种不同的仪器测量：双向激光多普勒测速仪（LDV）和DOCT。通过计算重复测量视网膜上级小动脉和小静脉的RBF的变异系数（CV），评估测量的重现性。在体外实验中，DOCT测量的流速与预设流速吻合良好。在体内，载瘤血管中的流量与两个子血管中的流量值之和一致。LDV和DOCT的RBF的平均CV值在小动脉和小静脉中均无显著性差异。新开发的分段扫描DOCT揭示了在体外玻璃毛细血管的测量的准确性和重复性的血液流速的测量在麻醉猫的视网膜小动脉和小静脉。
PURPOSE. To study retinal blood flow (RBF) measurement reproducibility using segmental-scanning Doppler optical coherence tomography (DOCT) in vitro in glass capillaries and in vivo in anesthetized cats.METHODS. As a preliminary study, the flow rates of human blood through glass capillaries were changed by using an infusion pump and measured at 13 preset velocities by DOCT. For in vivo measurement, the cats were anesthetized using sevoflurane. The flow in the parent vessel was compared with the sum of the flow values in the two daughter vessels. The RBF was measured using two different instruments: bidirectional laser Doppler velocimetry (LDV) and DOCT. The reproducibility of the measurements was assessed by calculating the coefficients of variation (CVs) for repeated measurements of RBF at the superior retinal arterioles and venules.RESULTS. In vitro, the flow velocities measured by DOCT agreed well with the preset velocities. In vivo, the flow in the parent vessel agreed with the sum of the flow values in the two daughter vessels. In addition, there were no significant differences in the mean averaged CVs of the RBF in both the arterioles and venules between LDV and DOCT.CONCLUSIONS. The newly developed segmental-scanning DOCT revealed the accuracy of the measurement in in vitro glass capillaries and reproducibility of the measurements of blood velocity in both the retinal arterioles and venules in anesthetized cats.