Adrm1, a putative cell adhesion regulating protein, is a novel proteasome-associated factor
Adrm1, a putative cell adhesion regulating protein, is a novel proteasome-associated factor
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DOI：
10.1016/j.jmb.2006.06.011
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发表时间：
2006-07-28
影响因子：
5.6
通讯作者：
Hartmann-Petersen, Rasmus
中科院分区：
文献类型：
--
作者：
Jorgensen, Jakob Ploug;Lauridsen, Anne-Marie;Hartmann-Petersen, Rasmus
我们已经确定Adrm1是26s蛋白酶体的调节性atp酶复合物的新组分：Adrm1与蛋白酶体的抗体沉淀，反之亦然。在凝胶过滤层析和非变性聚丙烯酰胺凝胶电泳上，Adrm1与蛋白酶体共迁移。Adrml已被描述为干扰素- γ诱导，110kda的重度糖基化膜蛋白。然而，我们在小鼠组织中发现Adrm1仅为42 kDa的肽，对应于非糖基化肽链的质量，并且不能在干扰素诱导HeLa细胞中产生。Adrml几乎完全存在于可溶性26s蛋白酶体中，尽管一小部分与膜相关，如蛋白酶体。Adrml在细胞中与26s蛋白酶体亚基S6a含量相当。HeLa细胞不含游离Adrml，但重组Adrml可以与细胞提取物中已有的26s蛋白酶体结合。Adrml可能与酵母蛋白酶体亚基Rpn13有远亲关系，据报道其突变体无明显表型。因此，HeLa细胞中Adrml的敲除对蛋白酶体的数量、体细胞蛋白的降解或多泛素化蛋白的积累没有影响。这表明Adrm1在蛋白酶体功能中具有特殊的作用。(c) 2006 Elsevier Ltd.版权所有。
We have identified Adrm1 as a novel component of the regulatory ATPase complex of the 26 S proteasome: Adrml was precipitated with an antibody to proteasomes and vice versa. Adrm1 co-migrated with proteasomes on gel-filtration chromatography and non-denaturing polyacrylamide gel electrophoresis. Adrml has been described as an interferon-gamma-inducible, heavily glycosylated membrane protein of 110 kDa. However, we found Adrm1 in mouse tissues only as a 42 kDa peptide, corresponding to the mass of the non-glycosylated peptide chain, and it could not be induced in HeLa cells with interferon. Adrml was present almost exclusively in soluble 26 S proteasomes, albeit a small fraction was membrane-associated, like proteasomes. Adrml was found in cells in amounts equimolar with S6a, a 26 S proteasome subunit. HeLa cells contain no pool of free Adrml but recombinant Adrml could bind to pre-existing 26 S proteasomes in cell extracts. Adrml may be distantly related to the yeast proteasome subunit Rpn13, mutants of which are reported to display no obvious phenotype. Accordingly, knock-down of Adrml in HeLa cells had no effect on the amount of proteasomes, or on degradation of bulk cell protein, or accumulation of polyubiquitinylated proteins. This indicates that Adrm1 has a specialised role in proteasome function. (c) 2006 Elsevier Ltd. All rights reserved.