Dura-evoked neck muscle activity involves purinergic and N-methyl-D-aspartate receptor mechanisms.
Dura-evoked neck muscle activity involves purinergic and N-methyl-D-aspartate receptor mechanisms.
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DOI：
10.1097/wnr.0000000000000489
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发表时间：
2015-12-16
期刊：
影响因子：
1.7
通讯作者：
Sessle BJ
中科院分区：
文献类型：
--
作者：
Yao D;Yoshida M;Sessle BJ
我们之前已经证明，包括额硬脑膜在内的颅面组织的有害刺激会反射性地引起颈部肌肉肌电图（EMG）活动的显著增加。本研究的主要目的是确定嘌呤能受体机制是否可能参与这些肌电效应，以及n -甲基-d-天冬氨酸（NMDA）受体过程是否调节嘌呤能机制。将P2×1， P2×3和P2×2/3受体激动剂α，β-亚甲基ATP（而非载体）应用于硬脑膜表面可引起同侧颈部肌电活动显著（P<0.05）增加，硬脑膜或鞘内应用选择性P2×1， P2×3和P2×2/3受体拮抗剂2 ',3 ' - o -（2,4,6-三硝基苯）ATP （TNPATP）可抑制该活性，但载体不能抑制该活性；鞘内应用2-氨基-5-磷酸戊酸（一种NMDA受体拮抗剂）也显著降低了硬脑膜应用α，β-亚甲基ATP引起的颈部肌电活动。这些数据表明，嘌呤能受体机制有助于前额硬脑膜的有害刺激反射性地引起颈部活动的增加，而髓质中的NMDA和嘌呤能受体机制可能调节这些嘌呤能相关的作用。
We have previously demonstrated that noxious stimulation of craniofacial tissues including the frontal dura reflexly evokes significant increases in neck muscle electromyographic (EMG) activity. The primary aim of this study was to determine whether purinergic receptor mechanisms may be involved in these EMG effects, and whether N-methyl-d-aspartate (NMDA) receptor processes modulate the purinergic mechanisms. Application of the P2×1, P2×3 and P2×2/3 receptor agonist α,β-methylene ATP (but not vehicle) to the dural surface evoked a significant (P<0.05) increase in ipsilateral neck EMG activity that could be suppressed by dural or intrathecal application of the selective P2×1, P2×3 and P2×2/3 receptor antagonist 2′,3′-O-(2,4,6-trinitrophenyl) ATP (TNPATP) but not by vehicle; the intrathecal application of 2-amino-5-phosphonopentanoic acid, an NMDA receptor antagonist, also significantly reduced the neck EMG activity evoked by dural application of α,β-methylene ATP. These data suggest that purinergic receptor mechanisms contribute to the increased neck activity that can be reflexly evoked by noxious stimulation of the frontal dura, and that NMDA as well as purinergic receptor mechanisms in the medulla may modulate these purinergic-related effects.
影响因子：
2.9
作者：
Watanabe, T.;Tsuboi, Y.;Sessle, B. J.;Iwata, K.;Hu, J. W.
通讯作者：
Hu, J. W.
影响因子：
7.4
作者：
Okuma, Hirohisa;Kitagawa, Yasuhisa;Takagi, Shigeharu
通讯作者：
Takagi, Shigeharu