Localization of the gene encoding the putative human HLA class II associated protein (PHAPI) to chromosome 15q22.3-q23 by fluorescence in situ hybridization.
Localization of the gene encoding the putative human HLA class II associated protein (PHAPI) to chromosome 15q22.3-q23 by fluorescence in situ hybridization.
复制标题
通过荧光原位杂交将编码假定的人类 HLA II 类相关蛋白 (PHAPI) 的基因定位于染色体 15q22.3-q23。
DOI：
10.1006/geno.1995.1257
复制
发表时间：
1995
期刊：
影响因子：
4.4
通讯作者：
M. Zimmer
中科院分区：
文献类型：
--
作者：
T. Fink;M. Vaesen;H. Kratzin;P. Lichter;M. Zimmer
假定的HLA II类相关蛋白PHAPI和PHAPII是根据它们与HLA Dr α链的胞质结构域结合的能力进行纯化和克隆的。它们可能是在免疫应答期间配体的细胞外结合后激活的跨膜信号传导途径的组分。这两种蛋白质在其C-末端区域共享延伸的高酸性氨基酸，这也表明了核定位。事实上，PHAPI很可能是大鼠富含亮氨酸的酸性核蛋白（LANP）（83.6%氨基酸同一性）的人类同源物，该蛋白显示位于浦肯野细胞的细胞核中。将该cDNA序列与EMBL数据库中的条目进行比较，发现PHAPII与一种名为SET的蛋白相同。SET基因位于染色体9 q34上，在一例急性未分化白血病（AUL）患者中被发现与推定的癌基因CAN融合。参考文献9篇，1图
The putative HLA class II associated proteins PHAPI and PHAPII were purified and cloned on the basis of their ability to bind to the cytoplasmatic domain of the HLA Dr{alpha}-chain. They might be components of the transmembrane signaling pathway that is activated after extracellular binding of ligands during the immune response. Both proteins share extended stretches of highly acidic amino acids in their C-terminal regions that also indicate a nuclear localization. Indeed, PHAPI is likely to be the human homologue of the rat {open_quotes}leucine-rich acidic nuclear protein{close_quotes} (LANP) (83.6% amino acid identity), which was shown to be localized in the nuclei of Purkinje cells. Comparison of the cDNA sequences with entries in the EMBL data library revealed that PHAPII is identical to a protein named SET. The SET gene is located on chromosome 9q34 and was found to be fused to the putative oncogene CAN in one patient with acute undifferentiated leukemia (AUL). 9 refs., 1 fig.