IMPROVED BACTERIAL TEST SYSTEM FOR DETECTION AND CLASSIFICATION OF MUTAGENS AND CARCINOGENS
IMPROVED BACTERIAL TEST SYSTEM FOR DETECTION AND CLASSIFICATION OF MUTAGENS AND CARCINOGENS
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DOI：
10.1073/pnas.70.3.782
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发表时间：
1973-01-01
影响因子：
11.1
通讯作者：
DURSTON, WE
中科院分区：
文献类型：
--
作者：
AMES, BN;LEE, FD;DURSTON, WE
我们先前描述了一组四株鼠伤寒沙门氏菌设计用于检测各种类型的诱变剂，并显示其在检测各种致癌物作为诱变剂的效用。通常包被这些细菌的脂多糖是诱变剂渗透到细胞膜的屏障。通过添加导致脂多糖缺陷的突变（rfa：深粗糙），对试验菌株组进行了改进。本文介绍了用这些菌株检测致突变物的技术，试验结果表明，它们是非常灵敏和方便的。阐明了移码突变的特异性。作为与四种菌株的测试的证明，我们描述了一个测试，比较了在深粗糙的菌株与无DNA切除修复的诱变性杀伤，并在缺乏切除修复的深粗糙的菌株使用正向诱变的测试。
We previously described a set of four strains ofSalmonella typhimuriumdesigned for detecting the various types of mutagens, and showed their utility in detecting a wide variety of carcinogens as mutagens. The lipopolysaccharide that normally coats these bacteria is a barrier to penetration of mutagens to the cell membrane. The set of tester strains has been improved by adding a mutation (rfa:deep rough) that results in a deficient lipopolysaccharide. The techniques for using these strains for detecting mutagens are presented and the tests are shown to be extremely sensitive and convenient. The specificity of frameshift mutagenesis is clarified. As adjuncts to the test with the four strains, we describe a test that compares mutagenic killing in deep rough strains with and without DNA excision repair, and a test using forward mutagenesis in a deep rough strain lacking excision repair.