INTERRELATIONSHIP OF BRAIN EDEMA, MOTOR DEFICITS, AND MEMORY IMPAIRMENT IN RATS EXPOSED TO FOCAL ISCHEMIA
INTERRELATIONSHIP OF BRAIN EDEMA, MOTOR DEFICITS, AND MEMORY IMPAIRMENT IN RATS EXPOSED TO FOCAL ISCHEMIA
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DOI：
10.1161/01.str.20.4.513
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发表时间：
1989-04-01
期刊：
影响因子：
8.3
通讯作者：
OHNISHI, ST
中科院分区：
文献类型：
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作者：
TOMINAGA, T;OHNISHI, ST
我们使用大鼠局灶性脑缺血模型研究了脑水肿、离子迁移、运动能力和记忆障碍的关系。通过结扎大脑中动脉和同侧颈总动脉并暂时闭塞对侧颈总动脉1小时来产生皮质梗死。测量含水量和钠、钾和钙浓度，直至缺血性损伤后第14天。观察到显著水肿形成;其在第3天达到峰值（p < 0.001），然后下降。组织钠浓度的变化方式与水分含量相似，但组织钾浓度的变化方式相反。早在缺血后第1天就检测到大量钙积累（几乎是正常水平的四倍）。钙浓度升高持续至第14天。在第3天进行的运动性能检查，包括斜面、平衡木和悬吊试验，显示运动能力显著降低（p < 0.001），甚至到第7天也没有恢复。在第2天进行被动回避学习，然后在第3天进行记忆保持测试。与假手术组相比，缺血组大鼠出现明显的记忆功能障碍（p < 0.001）。在第3天，在含水量和钙浓度之间获得了高相关系数（r = 0.91，p < 0.01，n = 13）。运动总分和被动回避反应的紊乱程度也与含水量有很好的相关性。
We investigated the relations of brain edema, ion shifts, motor performance, and memory impairment using a focal ischemia model in rats. Cortical infarction was produced by ligation of the middle cerebral artery and the ipsilateral common carotid artery combined with temporary occlusion of the contralateral common carotid artery for 1 hour. Water content and sodium, potassium, and calcium concentrations were measured until Day 14 after the ischemic insult. Significant edema formation was observed; it peaked on Day 3 (p < 0.001) and then declined. The tissue sodium concentration changed in a manner similar to that of water content, but the tissue potassium concentration changed in an opposite fashion. Massive accumulation of calcium was detected as early as Day 1 after ischemia (almost four times the normal level). The increased calcium concentration was sustained even up to Day 14. Motor performance examinations performed on Day 3, including inclined plane, balance beam, and prehensile tests, demonstrated significantly reduced (p < 0.001) motor ability that did not recover even by Day 7. Passive avoidance learning was carried out on Day 2, followed by a memory retention test on Day 3. Significant memory dysfunction was observed in ischemic compared with sham-operated rats (p < 0.001). A high correlation coefficient (r = 0.91, p < 0.01, n = 13) was obtained between water content and calcium concentration on Day 3. Both the total motor score and the degree of disturbance of the passive avoidance reaction also correlated well with water content.