A dual-modal red-emitting fluorescence probe for proteins based on modulation of AIE or TICT state
A dual-modal red-emitting fluorescence probe for proteins based on modulation of AIE or TICT state
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基于 AIE 或 TICT 状态调制的蛋白质双模态红光荧光探针
DOI：
10.1002/hc.21371
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发表时间：
2017
影响因子：
0.3
通讯作者：
Yang Changying
中科院分区：
文献类型：
--
作者：
Liu Chunlin;Yang Wei;Shen Ping;Gao Qingyun;Du Jinya;Yang Changying
合成了一种菁荧光团2‐[4‐N，N‐二苯基氨基苯基]‐β‐萘噻唑丙基磺酸盐N3。不对称proben3在DMSO/H2O混合物中表现出聚集诱导发射（AIE）的特性，而在二氧六烷/H2O混合物中表现出扭曲分子内电荷转移（TICT）的非发射状态。AIE和TICT状态均可被蛋白质调节，表现出开关效应。在含有90%水分数（fw）的DMSO/H2O混合物中，蛋白质分子吸附在聚集的n3晶体纳米颗粒上并扩散到表面，从而淬灭了n3的强荧光发射。蛋白质之间不同程度的猝灭表明蛋白质在n3聚集体颗粒上的吸附是由疏水和静电相互作用引起的。淬灭比（I0/I）与阳性蛋白溶菌酶（Lys）浓度呈Langmuir型曲线。在90%fw的二氧化硅/水混合物中，牛血清白蛋白（BSA）的加入导致非发射态TICT的减少，使n3的荧光发射开启。n3对牛血清白蛋白的荧光发射与其他蛋白（包括人血清白蛋白）的差异很大，这使我们能够建立一种灵敏的牛血清白蛋白探针。建立了一种ON - OFF和OFF - ON双峰红色荧光蛋白探针。
A cyanine fluorophore 2‐[4‐N,N‐diphenylaminostyryl]‐β‐naphthothiazolium propylsulfonate,N3, was synthesized. The asymmetric probeN3displayed aggregation‐induced emission (AIE) characteristic in DMSO/H2O mixtures, whereas it presented twisted intra‐molecular charge transfer (TICT) non‐emissive state in Dioxane/H2O (Diox/H2O) mixtures. Both the AIE and TICT state could be modulated by proteins, exhibiting switch effect. In DMSO/H2O mixtures with 90% water fractions (fw), protein molecules adsorbed upon the aggregated crystalline nanoparticles ofN3and spread over the surface, thus the strong fluorescence emission ofN3was quenched. The different degree of quenching among proteins revealed that the adsorption of proteins onN3aggregate particles arose from both hydrophobic and electrostatic interactions. The quenched ratio (I0/I) vs the concentration of positively protein lysozyme (Lys) gave a Langmuir type curve. In Diox/H2O mixtures with 90%fw, the addition of bovine serum albumin (BSA) led to the reduction of non‐emissive TICT state and made the fluorescence emission ofN3switch on. The large difference ofN3fluorescence emission toward BSA over other proteins including human serum albumin allowed us to establish a sensitive probe for BSA. An ON‐OFF and OFF‐ON dual‐modal red‐emitting fluorescence probe for proteins was established.