Deep sequencing in cancer research.
Deep sequencing in cancer research.
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DOI：
10.1093/jjco/hys206
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发表时间：
2013-02
影响因子：
2.4
通讯作者：
Kenichi Yoshida;M. Sanada;S. Ogawa
中科院分区：
文献类型：
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作者：
Kenichi Yoshida;M. Sanada;S. Ogawa
癌症是由细胞基因组的改变引起的，包括单核苷酸变异、小插入和小缺失(INDel)、拷贝数变化和其他结构变异，因此，全面检测它们对于全面了解癌症的发病机制、提高诊断水平以及开发新的治疗方法具有重要意义。从这一观点来看，大规模并行(或下一代)测序技术的最新发展为实现这一需求提供了前所未有的可能性，因为它能够对癌细胞的整个基因组进行单核苷酸解析分析，并对编码序列或转录体进行更有针对性的分析。通过国际合作，现在已经使用这些技术分析了各种各样的癌细胞类型，以帮助揭示它们的发病机制。在这篇综述中，我们简要概述了通过对癌症基因组测序的巨大努力而获得的癌症研究的最新进展。
Cancer is caused by alterations in the cellular genome including single-nucleotide variations, small insertions and deletions (indels), copy number changes and other structural variations and, as such, their detection in a comprehensive manner is of critical importance for fully understanding cancer pathogenesis, improvement of diagnosis as well as the development of novel therapeutics. In this point of views, the recent development of massively parallel (or 'next-generation') sequencing technologies has provided an unprecedented possibility to accomplish this need by enabling single-nucleotide resolution analysis of the entire genome of cancer cells as well as more targeted analysis of coding sequencing or transcriptomes. Through international co-operations, a wide variety of cancer cell types have now been analyzed using these technologies to help unmask their pathogenesis. In this review, we briefly overview the recent advances in cancer research obtained through the massive effort of sequencing cancer genomes.