Vitellogenin uptake activity in the intestinal ducts of intraovarian embryos in a viviparous teleost Xenotoca eiseni
Vitellogenin uptake activity in the intestinal ducts of intraovarian embryos in a viviparous teleost Xenotoca eiseni
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DOI：
10.1016/j.bbrc.2023.01.009
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发表时间：
2023-01-10
影响因子：
3.1
通讯作者：
Iida，Atsuo
中科院分区：
文献类型：
--
作者：
Nomura，Jumpei;Yokoi，Hayato;Iida，Atsuo
在属于古迪科的胎生硬骨鱼物种中，卵巢内胚胎在妊娠期生长时吸收母体补充物。它们通过滋养带（一种后肠来源的胎盘结构）吸收这些成分。我们以前对鹅口疮Xenotoca eiseni的研究表明，卵巢内胚胎通过滋养带吸收卵黄蛋白原（Vtg）。但另一组表明卵黄成分在胚胎肠腔中积累。在这里，我们调查了肠道是否能够吸收蛋白质，因为是营养带绦虫。免疫组织化学研究表明，内源性卵黄蛋白原检测到肠上皮细胞的卵巢内胚胎。使用FITC-Vtg的示踪剂分析也表明肠组织可以摄取蛋白质。内吞相关基因在营养带绦虫中的表达也在胚胎肠组织中检测到。脂质转运蛋白基因在滋养带绦虫中不表达，但在胚胎肠中检测到。这一证据表明，卵巢内的胚胎具有两个不同的网站摄取母体蛋白。然而，胚胎肠和滋养带绦虫的假定功能可能不相同。这项研究提供了一个新的视角，如何母-胚的matrotrophic相互作用发生了变化，在胎生硬骨鱼的进化。
In the viviparous teleost species belonging to the family Goodeidae, intraovarian embryos absorb maternal supplements while they grow during the gestation period. They take up the components via trophotaeniae, a hindgut-derived placental structure. Our previous study using a goodeid speciesXenotoca eisenirevealed that intraovarian embryos absorb the yolk protein vitellogenin (Vtg) via the trophotaenia. However, another group indicated yolk components accumulate in the intestinal lumen ofX. eiseniembryos. Here, we investigated whether the intestinal duct is capable of protein uptake, as is the trophotaenia. Immunohistochemical studies indicated that endogenous vitellogenin is detected in the intestinal epithelial cells of the intraovarian embryo. Tracer analysis using FITC-Vtg also indicated that intestinal tissues can take up protein. The endocytosis-related genes expressed in trophotaenia were also detected in the intestinal tissues of the embryo. Lipid transporter genes which are not expressed in the trophotaenia were detected in the embryonic intestine. This evidence suggests that the intraovarian embryo ofX. eisenipossesses two distinct sites for uptake of the maternal proteins. However, the presumed functions of the embryonic intestine and trophotaenia might be not identical. The study provides a new perspective on how mother-to-embryo matrotrophic interactions have changed in the evolution of viviparous teleosts.