Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm.
Multimodal Nonlinear Microscopy by Shaping a Fiber Supercontinuum From 900 to 1160 nm.
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DOI：
10.1109/jstqe.2011.2168559
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Boppart SA
中科院分区：
文献类型：
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作者：
Liu Y;Tu H;Benalcazar WA;Chaney EJ;Boppart SA
非线性显微镜在生物光子成像中得到了广泛的应用。脉冲整形提供了对超快脉冲的非线性光学过程的控制，用于选择性成像和对比度增强。在这项研究中，非线性显微镜，包括双光子荧光，二次谐波产生，和三次谐波产生，使用从光纤超连续谱（SC）从900至1160 nm的脉冲形状进行。利用空间光调制器对Yb：KYW脉冲激光耦合到光子晶体光纤中产生的超连续谱进行了光谱滤波和压缩。成形脉冲用于细胞和组织样品的非线性光学成像。光纤SC的振幅和相位整形功能可通过单光束和易于调谐的设置在宽带宽范围内提供选择性和高效的非线性光学成像。
Nonlinear microscopy has become widely used in biophotonic imaging. Pulse shaping provides control over nonlinear optical processes of ultrafast pulses for selective imaging and contrast enhancement. In this study, nonlinear microscopy, including two-photon fluorescence, second harmonic generation, and third harmonic generation, was performed using pulses shaped from a fiber supercontinuum (SC) spanning from 900 to 1160 nm. The SC generated by coupling pulses from a Yb:KYW pulsed laser into a photonic crystal fiber was spectrally filtered and compressed using a spatial light modulator. The shaped pulses were used for nonlinear optical imaging of cellular and tissue samples. Amplitude and phase shaping the fiber SC offers selective and efficient nonlinear optical imaging over a broad bandwidth with a single-beam and an easily tunable setup.