Post‐transcriptional regulation through the HO 3′‐UTR by Mpt5, a yeast homolog of Pumilio and FBF
Post‐transcriptional regulation through the HO 3′‐UTR by Mpt5, a yeast homolog of Pumilio and FBF
复制标题
Mpt5（Pumilio 和 FBF 的酵母同源物）通过 HO 3-UTR 进行转录后调控
DOI：
10.1093/emboj/20.3.552
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Irie
中科院分区：
文献类型：
--
作者：
Tomofumi Tadauchi;Kunihiro Matsumoto;I. Herskowitz;K. Irie
Drosophila Pumilio (Pum) and Caenorhabditis elegans FBF bind to the 3′‐untranslated region (3′‐UTR) of their target mRNAs and repress translation. Pum and FBF are members of a large and evolutionarily conserved protein family, the Puf family, found in Drosophila, C.elegans, humans and yeasts. Budding yeast, Saccharomyces cerevisiae, has five proteins with conserved Puf motifs: Mpt5/Uth4, Ygl014w, Yll013c, Jsn1 and Ypr042c. Here we report that Mpt5 negatively regulates expression of the HO gene. Loss of MPT5 increased expression of reporter genes integrated into the ho locus, whereas overexpression of MPT5 decreased expression. Repression required the 3′‐UTR of HO, which contains a tetranucleotide, UUGU, also found in the binding sites of Pum and FBF. Mutation of UUGU to UACU in the HO 3′‐UTR abolished Mpt5‐mediated repression. Studies using a three‐hybrid assay for RNA binding indicate that Mpt5 binds to the 3′‐UTR of HO mRNA containing a UUGU sequence but not a UACU sequence. These observations suggest that the yeast Puf homolog, Mpt5, negatively regulates HO expression post‐ transcriptionally.
登录
查看更多内容
影响因子：
3.3
作者：
H. Hata;H. Mitsui;Hong Liu;Yongli Bai;C. Denis;Y. Shimizu;A. Sakai
通讯作者：
H. Hata;H. Mitsui;Hong Liu;Yongli Bai;C. Denis;Y. Shimizu;A. Sakai
影响因子：
2.9
作者：
P. Zamore;D. Bartel;R. Lehmann;J. Williamson
通讯作者：
P. Zamore;D. Bartel;R. Lehmann;J. Williamson
影响因子：
3.3
作者：
P. James;J. Halladay;E. Craig
通讯作者：
P. James;J. Halladay;E. Craig
DOI：
--
发表时间：
1996
期刊：
--
影响因子：
--
作者：
F. Sherman
通讯作者：
F. Sherman
DOI：
10.1073/pnas.93.16.8496
发表时间：
1996-08-06
影响因子：
11.1
作者：
SenGupta, DJ;Zhang, BL;Wickens, M
通讯作者：
Wickens, M