Maternal vitamin D predominates over genetic factors in determining neonatal circulating vitamin D concentrations
Maternal vitamin D predominates over genetic factors in determining neonatal circulating vitamin D concentrations
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DOI：
10.3945/ajcn.112.035683
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发表时间：
2012-07-01
影响因子：
7.1
通讯作者：
Saffery, Richard
中科院分区：
文献类型：
--
作者：
Novakovic, Boris;Galati, John C.;Saffery, Richard
背景：环境、遗传和表观遗传起源的新生儿维生素 D 状态有多种潜在调节因素。这些因素与新生儿循环维生素 D 之间的关系尚未完全确定。 目的：本研究的目的是检查遗传因素、母体循环 25-羟基维生素 D [25(OH)D] 浓度以及编码活性维生素 D 主要分解代谢酶 [25(OH)D-24-羟化酶，由 CYP24A1 编码] 的基因的胎盘甲基化水平对新生儿 25(OH)D 的相对贡献设计：我们使用经典的双胞胎研究设计来确定新生儿 25(OH)D 的遗传贡献。本研究共纳入 86 对双胞胎（32 对同卵双胞胎和 54 对异卵双胞胎）。使用25(OH)D试剂盒测定血清25(OH)D。通过基质辅助激光解吸飞行时间质谱法测量 CYP24A1 启动子 DNA 甲基化。结果：母体和新生儿 25(OH)D 显示出很强的关联性 (R-2 = 0.19)。单卵和双卵配对内血清 25(OH)D 相关性相似（R-2 分别 = 0.71 和 0.67），这表明没有遗传效应。胎盘 CYP24A1 甲基化并未显示与母体或新生儿 25(OH)D 浓度相关。结论：我们的结果表明，母体循环 25(OH)D 是新生儿循环 25(OH)D 浓度最重要的调节因子，潜在的遗传因素发挥的作用有限。 CYP24A1 启动子的胎盘甲基化似乎受到遗传影响，但没有证据表明该基因的甲基化水平与循环母体或新生儿 25(OH)D 之间存在明显关系。美国临床营养杂志 2012； 96：188-95。
Background: There are multiple potential regulators of neonatal vitamin D status of environmental, genetic, and epigenetic origins. The relation between these factors and circulating neonatal vitamin D has yet to be fully characterized.Objective: The aim of this study was to examine the relative contribution of genetic factors, maternal circulating 25-hydroxyvitamin D [25(OH)D] concentrations, and the placental methylation level of the gene that encodes the primary catabolic enzyme of active vitamin D [25(OH)D-24-hydroxylase encoded by CYP24A1] to neonatal 25(OH)D concentrations.Design: We used the classical twin study design to determine the genetic contribution to neonatal 25(OH)D. A total of 86 twin pairs (32 monozygotic and 54 dizygotic twin pairs) were included in this study. Serum 25(OH)D was measured by using a 25(OH)D kit. CYP24A1 promoter DNA methylation was measured by means of matrix-assisted laser desorption time-of-flight mass spectrometry.Results: Maternal and neonatal 25(OH)D showed a strong association (R-2 = 0.19). Monozygotic and dizygotic within-pair serum 25(OH)D correlations were similar (R-2 = 0.71 and 0.67, respectively), which suggested no genetic effect. Placental CYP24A1 methylation did not show an association with maternal or neonatal 25(OH)D concentrations.Conclusions: Our results suggest that maternal circulating 25(OH)D is the most significant regulator of neonatal circulating 25(OH)D concentrations, with underlying genetic factors playing a limited role. The placental methylation of the CYP24A1 promoter appears subject to a genetic influence, although no evidence of a relation between the methylation level of this gene and circulating maternal or neonatal 25(OH)D was apparent. Am J Clin Nutr 2012; 96:188-95.