Uniform olivocerebellar conduction time underlies Purkinje cell complex spike synchronicity in the rat cerebellum.
Uniform olivocerebellar conduction time underlies Purkinje cell complex spike synchronicity in the rat cerebellum.
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均匀的橄榄小脑传导时间是大鼠小脑中浦肯野细胞复合体尖峰同步性的基础。
DOI：
10.1113/jphysiol.1993.sp019857
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Llinás,R
中科院分区：
文献类型：
--
作者：
Sugihara,I;Lang,EJ;Llinás,R
1. 通过潜伏期测量、多电极记录和Phaseolus vulgaris白细胞凝集素（PHA - L）追踪成年大鼠攀爬纤维，研究了小脑脊液纤维传导时间的等时性问题，并以此作为同步小脑浦肯野细胞复杂峰活动的基础。2. 通过记录来自小脑不同区域的浦肯野细胞复合体脉冲（CS）反应来测量小脑脊液纤维的传导时间。通过刺激下橄榄附近的橄榄小脑纤维可诱发csp。尽管长度不同，但通过光学显微镜直接测定，不同攀爬纤维的传导时间相当均匀，为3.98 +/‐0.36 ms（平均+/‐s.d., n = 660）。3. 采用多电极记录自发性浦肯野细胞CS活动的方法研究了小脑皮层CS同步性的空间范围。CS记录来自位于小叶小腿2a表面和沿壁的浦肯野细胞。浦肯野细胞中同时（在1ms内）CS活动的背侧-尾侧带状分布向下延伸到记录的最深区域（1.6 - 2.6 mm深）。如以往实验所示，同时CS活动的分布在小脑皮层的中外侧轴上没有明显延伸（500微米）。4. 在两只动物中，通过向对侧下橄榄注入PHA - L染色纤维，详细测定了橄榄小脑纤维束的长度。该测量包括终止于26个不同区域的纤维束，范围从各种叶的顶部到半球和蚓部裂缝的底部。经组织收缩校正后，测量到的最长纤维束长度（15.8 mm，终止于小叶6b， a区）与最短纤维束长度（8.3 mm，终止于初级裂缝底部皮层，D区）相差47.5%。为了获得等时传导时间，计算出这两束纤维的传导速度分别为4.22 m/s和2.37 m/s。5. 通过在测量点之间进行插值，得出了一个简单的公式来估计终止于小脑皮层任何给定区域的橄榄小脑纤维的平均长度，不包括小叶旁、小叶和半球最外侧的区域。6. 我们研究了传导速度随长度变化可能导致全球等时性的最可能机制。（摘要删节为400字）
1. The issue of isochronicity of olivocerebellar fibre conduction time as a basis for synchronizing complex spike activity in cerebellar Purkinje cells has been addressed by latency measurement, multiple‐electrode recording and Phaseolus vulgaris leucoagglutinin (PHA‐L) tracing of climbing fibres in the adult rat. 2. The conduction time of the olivocerebellar fibres was measured by recording Purkinje cell complex spike (CS) responses from various areas of the cerebellum. The CSs were evoked by stimulating the olivocerebellar fibres near the inferior olive. In spite of a difference in length, as determined directly by light microscopy, the conduction times of different climbing fibres were quite uniform, 3.98 +/‐ 0.36 ms (mean +/‐ S.D., n = 660). 3. Multiple‐electrode recording of spontaneous Purkinje cell CS activity was employed to study the spatial extent of CS synchronicity in the cerebellar cortex. Recordings of CS were obtained from Purkinje cells located on the surface and along the walls of lobule crus 2a. The rostrocaudal band‐like distribution of simultaneous (within 1 ms) CS activity in Purkinje cells extended down the sides of the cerebellar folia to the deepest areas recorded (1.6‐2.6 mm deep). As shown in previous experiments, the distribution of simultaneous CS activity did not extend significantly (500 microns) in the mediolateral axis of the cerebellar cortex. 4. In two animals a detailed determination of the length of the olivocerebellar fibre bundles was performed by staining the fibres with PHA‐L injected into the contralateral inferior olive. This measurement included fibre bundles terminating in twenty‐six different areas, ranging from the tops of the various folia to the bottoms of the fissures in both the hemisphere and the vermis. There was a 47.5% difference between the length of the longest measured fibre bundle (15.8 mm, terminating in lobule 6b, zone A) and the length of the shortest measured fibre bundle (8.3 mm, terminating in the cortex at the base of the primary fissure, zone D), after correction for tissue shrinkage. To attain an isochronous conduction time the conduction velocities for these two fibre bundles were calculated to be 4.22 m/s and 2.37 m/s, respectively. 5. By interpolating between measured points a simple formula was derived to estimate the average length of olivocerebellar fibres terminating in any given area of the cerebellar cortex, excluding the paraflocculus, the flocculus and the most lateral regions of the hemisphere. 6. We investigated the most likely mechanisms by which conduction velocity variations with length could result in global isochronicity.(ABSTRACT TRUNCATED AT 400 WORDS)
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猫下橄榄对小脑和大脑皮层刺激的反应
DOI：
10.1113/jphysiol.1966.sp008108
发表时间：
1966
期刊：
The Journal of Physiology
影响因子：
--
作者：
D. Armstrong;R. Harvey
通讯作者：
R. Harvey
可修饰突触的分类及其在调节模型中的应用
DOI：
10.1098/rspb.1967.0070
发表时间：
1967
期刊：
Proceedings of the Royal Society of London. Series B. Biological Sciences
影响因子：
--
作者：
G. Brindley
通讯作者：
G. Brindley
下橄榄轴突的分支终止于小脑的不同叶、小叶或叶。
影响因子：
2.9
作者：
D. Armstrong;R. Harvey;R. F. Schild
通讯作者：
R. F. Schild
用菜豆白细胞凝集素（PHA-L）对大鼠橄榄小脑系统进行顺行追踪，演示小脑核的攀爬纤维侧支神经支配。
DOI：
--
发表时间：
1989
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
J. Want;L. Wiklund;M. Guégan;And T. RUIGROK;J. Voogd
通讯作者：
J. Voogd
影响因子：
2.5
作者：
ROSSI, F;WIKLUND, L;STRATA, P
通讯作者：
STRATA, P