Strain-specific quantification of Wolbachia density in Aedes albopictus and effects of larval rearing conditions
Strain-specific quantification of Wolbachia density in Aedes albopictus and effects of larval rearing conditions
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DOI：
10.1111/j.0962-1075.2004.00490.x
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发表时间：
2004-06-01
影响因子：
2.6
通讯作者：
Sinkins, SP
中科院分区：
文献类型：
--
作者：
Dutton, TJ;Sinkins, SP
内共生体沃尔巴克氏体的密度可以影响被称为细胞质不相容性（CI）的交叉不育的表达，以及其母体传播率。白纹伊蚊含有沃尔巴克氏菌菌株 wAlbA 和 wAlbB 的双重感染。开发了一种菌株特异性实时定量 PCR 检测方法，用于量化个体蚊子内沃尔巴克氏体菌株的相对密度。 wAlbB 菌株的密度始终高于 wAlbA，这可以解释 wAlbA 的母体传播率略低的原因。还检查了幼虫拥挤和营养应激的影响。幼虫拥挤总是会减小成虫的体型，但只有当拥挤伴随着有限的营养供应时，相对于空闲条件，沃尔巴克氏体菌株的密度才会降低。然而，拥挤的饲养条件从未导致品系分离或 CI 外显率降低。母体传播率和 CI 外显率是决定相对沃尔巴克氏体种群入侵动态的两个最重要的变量，并且两者都比模拟果蝇模型系统中报道的要高得多。
The density of the endosymbiont Wolbachia can influence the expression of the crossing sterilities known as cytoplasmic incompatibility (CI), and also its rate of maternal transmission. Aedes albopictus mosquitoes contain a superinfection with the Wolbachia strains wAlbA and wAlbB. A strain-specific real-time quantitative PCR assay was developed and used to quantify relative Wolbachia strain densities within individual mosquitoes. The wAlbB strain was consistently found to be at higher density than wAlbA, which can explain a slightly lower rate of maternal transmission reported for wAlbA. The effects of larval crowding and nutritional stress were also examined. Larval crowding always reduced adult size, but reduced the density of Wolbachia strains relative to uncrowded conditions only if crowding was accompanied by restricted nutrient availability. Crowded rearing conditions never resulted in strain segregation or in a reduction in the penetrance of CI, however. The rate of maternal transmission and the penetrance of CI are the two most important variables that determine relative Wolbachia population invasion dynamics, and both are considerably higher here than have been reported in the Drosophila simulans model system.