Peripheral B cell maturation. II. Heat-stable antigen(hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells.
Peripheral B cell maturation. II. Heat-stable antigen(hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells.
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DOI：
10.4049/jimmunol.151.9.4431
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发表时间：
1993-11
影响因子：
4.4
通讯作者：
D. Allman;S. Ferguson;V. Lentz;M. Cancro
中科院分区：
文献类型：
--
作者：
D. Allman;S. Ferguson;V. Lentz;M. Cancro
HSAhi B 细胞占成年小鼠脾 B 细胞的 5% 至 10%，并且表型和功能不成熟。为了评估它们的起源以及与成熟、热稳定的 Ag (HSA)lo B 细胞的关系，我们测定了脾 B 细胞在整个发育过程中以及在给予成人 B 耗尽骨髓的致命辐射成人的重建过程中的 HSA 和表面 IgD 表型。在每种情况下，HSAhi 脾 B 细胞在 B 细胞发生的最早阶段占主导地位。此外，5-溴-2'-脱氧尿苷标记实验表明骨髓和外周 HSAhi 池内的快速更新，过继转移研究表明外周 HSAhi 脾 B 细胞在 4 天内分化为 HSAlo。最后，脾脏 HSAhi B 细胞重建初级体液反应和记忆体液反应。总之，这些数据表明 HSAhi 亚群中的脾 B 细胞处于成人外周的中间成熟阶段。
HSAhi B cells comprise 5 to 10% of adult mouse splenic B cells and are phenotypically and functionally immature. To assess their origin and relationship to mature, heat-stable Ag (HSA)lo B cells, we determined HSA and surface IgD phenotype among splenic B cells throughout development, as well as during reconstitution of lethally irradiated adults given adult B-depleted bone marrow. In each case, HSAhi splenic B cells predominate during the earliest stages of B cell genesis. Furthermore, 5-bromo-2'-deoxyuridine labeling experiments indicate rapid turnover within both the marrow and peripheral HSAhi pools, and adoptive transfer studies show that peripheral HSAhi splenic B cells differentiate to HSAlo within 4 days. Finally, splenic HSAhi B cells reconstitute both primary and memory humoral responses. Together, these data indicate that splenic B cells in the HSAhi subset are an intermediate maturational stage in the adult periphery.