A new test paradigm for social recognition evidenced by urinary scent marking behavior in C57BL/6J mice
A new test paradigm for social recognition evidenced by urinary scent marking behavior in C57BL/6J mice
复制标题
DOI：
10.1016/j.bbr.2008.02.009
复制
发表时间：
2008-06-26
影响因子：
2.7
通讯作者：
Blanchard, Robert J.
中科院分区：
文献类型：
--
作者：
Arakawa, Hiroyuki;Arakawa, Keiko;Blanchard, Robert J.
嗅觉是小鼠种内识别和交流的主要感觉元素。本研究调查了高度近交的C57BL/6J(C57)品系雄性个体的气味标记行为，以评估这些行为对社会熟悉度和对社会信号的反应的清晰和一致的衡量标准。当C57雄虫被放置在一个房间里时，他们会进行气味标记，并用金属丝网隔开它们与同种动物。雄性小鼠(C57或杂交的CD-1小鼠)表现出快速适应气味标记(在试验中减少标记)，每隔24小时重复暴露于C57或CD-1品系的刺激动物，或暴露在空舱中。随后暴露在基因不同的新小鼠(CD-1暴露后的CD-1，或C57暴露后的CD-1)或新环境(不同形状的腔室)中，可以恢复标记，而对新的但基因相同的小鼠(C57暴露后的C57)或空腔的反应不会。这一发现表明，雄性小鼠通过气味标记的习惯化和恢复来区分熟悉的和新的同种异物，但C57和CD-1小鼠都不能区分新的和熟悉的C57雄性小鼠；可能是因为它们的气味模式相似。数据还表明，气味标记可以区分新的和熟悉的语境。(C)2008爱思唯尔B.V.保留所有权利。
Olfaction is a major sensory element in intraspecies recognition and communication in mice. The present study investigated scent marking behaviors of males of the highly inbred C57BL/6J (C57) strain in order to evaluate the ability of these behaviors to provide clear and consistent measures of social familiarity and response to social signals. C57 males engage in scent marking when placed in a chamber with a wire mesh partition separating them from a conspecific. Male mice (C57 or outbred CD-1 mice) showed rapid habituation of scent marking (decreased marking over trials) with repeated exposure at 24-h intervals, to a stimulus animal of the C57 or CD-1 strains, or to an empty chamber. Subsequent exposure to a genetically different novel mouse (CD-1 after CD-1 exposure, or CD-1 after C57 exposure) or to a novel context (different shaped chamber) produced recovery of marking, while responses to a novel but genetically identical mouse (C57 after C57 exposure) or to the empty chamber did not. This finding demonstrated that male mice differentiate familiar and novel conspecifics as expressed by habituation and recovery of scent marking, but neither C57 or CD-1 mice can differentiate new vs. familiar C57 males; likely due to similarities in their odor patterns. The data also indicate that scent marking can differentiate novel from familiar contexts. (c) 2008 Elsevier B.V. All rights reserved.