Estrogenic activity of phenol red in rat anterior pituitary cells in culture.
Estrogenic activity of phenol red in rat anterior pituitary cells in culture.
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DOI：
10.1016/s0006-291x(86)80125-5
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发表时间：
1986-12
影响因子：
3.1
通讯作者：
J. Hubert;A. Vincent;F. Labrie
中科院分区：
文献类型：
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作者：
J. Hubert;A. Vincent;F. Labrie
以大鼠垂体前叶细胞为研究对象，研究了广泛应用于细胞培养基的pH指示剂酚红的雌激素活性。40 μM苯酚红孵育72 h后，催乳素细胞含量增加40 - 50%，促黄体生成素释放激素刺激促黄体生成素释放100%。与抗雌激素LY156758同时孵育可完全逆转这两种作用。在大鼠子宫[3H]雌二醇结合实验中，苯酚红在浓度大于10 μM时出现明显位移，而在常用培养基中其浓度约为40 μM。从目前的结果，我们得出结论，酚红在正常组织中作为弱雌激素，在使用雌激素敏感细胞或组织培养的研究中应考虑其雌激素活性。
The estrogenic activity of phenol red, a pH indicator widely used in cell culture media, was studied in rat anterior pituitary cells. After 72 hours of incubation with 40 μM phenol red, a 40–50% increase in prolactin cell content and a 100% stimulation of luteinizing hormone-releasing hormone induced luteinizing hormone release was observed. Both effects could be completely reversed by simultaneous incubation with the antiestrogen LY156758. In the rat uterine [3H] estradiol binding assay, phenol red showed a significant displacement at concentrations above 10 μM while its concentration in the commonly used culture media is about 40 μM. From the present results, we conclude that phenol red acts as a weak estrogen in normal tissues and that its estrogenic activity should be taken into account in studies using estrogen-sensitive cell or tissue cultures.