Migration and accumulation of eosinophils toward regional lymph nodes after airway allergen challenge
Migration and accumulation of eosinophils toward regional lymph nodes after airway allergen challenge
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DOI：
10.1016/j.jaci.2004.08.011
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发表时间：
2004-10-01
影响因子：
14.2
通讯作者：
Gelfand, EW
中科院分区：
文献类型：
--
作者：
Duez, C;Dakhama, A;Gelfand, EW
背景：嗜酸性粒细胞在过敏性呼吸道炎症中起主要作用，因为它们能够释放有毒介质。目的：在本研究中，我们评估了致敏后嗜酸性粒细胞向胸腺淋巴结的迁移，并分析了与抗原呈递有关的分子的表达。方法：分别于第1天和第10天经腹注射卵白蛋白致敏小鼠，第20天经鼻腔注射卵白蛋白1次，观察血液、肺组织匀浆、支气管肺泡灌洗液和TLN中嗜酸粒细胞的动态变化。结果：TLN、肺组织和血液中嗜酸性粒细胞募集的动力学相似，在致敏后12h开始，48h达到高峰。大约70%的TLN嗜酸性粒细胞表达MHC II类分子，相比之下，血液和肺中的这一比例不到25%。此外，与血液和肺嗜酸粒细胞相比，TLN嗜酸性粒细胞表达更高水平的MHC-II和CD86。大多数嗜酸性粒细胞表达CD80和CD54，只有少数嗜酸性粒细胞表达CD40。结论：致敏小鼠变应原攻击后早期TLN中存在不同表型的嗜酸性粒细胞，支持其作为抗原提呈细胞的能力，维持呼吸道的过敏/炎症反应。
Background: Eosinophils play a major role in allergic airway inflammation because of their ability to release toxic mediators. In addition, they are able to migrate toward draining thoracic lymph nodes (TLNs) after intratracheal administration, where they can function as antigen-presenting cells.Objective: In this study, we evaluated in vivo eosinophil migration toward the TLN after allergen sensitization and analyzed expression of molecules involved in antigen presentation.Methods: Mice were sensitized by intraperitoneal injection of ovalbumin on days 1 and 10 and challenged once intranasally with ovalbumin on day 20. The kinetics of eosinophilia was evaluated in blood, lung tissue homogenate, bronchoalveolar lavage fluid, and TLN. Cell surface staining was analyzed by flow cytometry.Results: The kinetics of eosinophil recruitment was similar in TLN, lung tissue, and blood, beginning at 12 hours and peaking at 48 hours after allergen challenge. Approximately 70% of TLN eosinophils expressed MHC class II molecules, compared with less than 25% in blood and lungs. Moreover, TLN eosinophils expressed higher levels of MHC class II and CD86 compared with blood and lung eosinophils. Most eosinophils expressed CD80 and CD54, whereas only a few eosinophils expressed CD40. Eosinophils in lungs and TLN appeared to be activated with lower CD62-ligand expression compared with blood eosinophils.Conclusion: The presence of eosinophils with a different phenotype in the TLN at early time points after allergen challenge of sensitized mice supports their capacity to serve as antigen-presenting cells, sustaining allergic/inflammatory responses in the airways.