An orthologue of the kit-related gene fms is required for development of neural crest-derived xanthophores and a subpopulation of adult melanocytes in the zebrafish, Danio rerio.
An orthologue of the kit-related gene fms is required for development of neural crest-derived xanthophores and a subpopulation of adult melanocytes in the zebrafish, Danio rerio.
复制标题
DOI：
--
复制
发表时间：
2000-07
期刊：
影响因子：
4.6
通讯作者：
D. Parichy;D. Ransom;B. Paw;Len I Zon;Stephen L. Johnson
中科院分区：
文献类型：
--
作者：
D. Parichy;D. Ransom;B. Paw;Len I Zon;Stephen L. Johnson
在脊椎动物幼体和成体中表达的特征的发育机制在很大程度上仍然未知。鱼类的色素模式提供了一个机会，以确定胚胎后形态发生和分化所需的基因和细胞行为。在斑马鱼（Danio rerio）中，色素模式反映了三类神经嵴衍生色素细胞的空间排列：黑色黑素细胞、黄色黄色素细胞和银虹膜色素细胞。我们证明了D. rerio色素模式突变体豹消除了胚胎和成体中的黄色素细胞，并且在黑素细胞条纹的成体模式的发育中具有缺陷。我们发现，豹对应的c-fms基因，它编码的III型受体酪氨酸激酶，是最接近已知的同源物先前确定的色素模式基因，试剂盒的直向同源物。在小鼠中，fms对于巨噬细胞和破骨细胞谱系的发育是必需的，但与神经嵴或色素细胞的发育无关。相反，我们的分析表明，fms是由D. rerio xanthophore前体和fms促进黑素细胞死亡和迁移的正常模式在成年条纹形成。最后，我们表明，fms是需要一个晚期发展，套件独立的成年黑素细胞亚群的外观。这些发现揭示了fms在色素模式发育中意想不到的作用，并证明了平行的神经嵴衍生的色素细胞群依赖于两个基本上同源的基因kit和fms的活性。
Developmental mechanisms underlying traits expressed in larval and adult vertebrates remain largely unknown. Pigment patterns of fishes provide an opportunity to identify genes and cell behaviors required for postembryonic morphogenesis and differentiation. In the zebrafish, Danio rerio, pigment patterns reflect the spatial arrangements of three classes of neural crest-derived pigment cells: black melanocytes, yellow xanthophores and silver iridophores. We show that the D. rerio pigment pattern mutant panther ablates xanthophores in embryos and adults and has defects in the development of the adult pattern of melanocyte stripes. We find that panther corresponds to an orthologue of the c-fms gene, which encodes a type III receptor tyrosine kinase and is the closest known homologue of the previously identified pigment pattern gene, kit. In mouse, fms is essential for the development of macrophage and osteoclast lineages and has not been implicated in neural crest or pigment cell development. In contrast, our analyses demonstrate that fms is expressed and required by D. rerio xanthophore precursors and that fms promotes the normal patterning of melanocyte death and migration during adult stripe formation. Finally, we show that fms is required for the appearance of a late developing, kit-independent subpopulation of adult melanocytes. These findings reveal an unexpected role for fms in pigment pattern development and demonstrate that parallel neural crest-derived pigment cell populations depend on the activities of two essentially paralogous genes, kit and fms.