Influence of glutamine and vitamin E on growth and antioxidant capacity of fish enterocytes
Influence of glutamine and vitamin E on growth and antioxidant capacity of fish enterocytes
复制标题
谷氨酰胺和维生素E对鱼肠上皮细胞生长和抗氧化能力的影响
DOI：
10.1111/j.1365-2095.2008.00605.x
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发表时间：
2009-08-01
影响因子：
3.5
通讯作者：
Feng, L.
中科院分区：
文献类型：
--
作者：
Jiang, J.;Zheng, T.;Feng, L.
本研究探讨了谷氨酰胺和维生素E油对离体鱼类心肌细胞生长和抗氧化能力的影响。分别用含0、2.0、4.7. 6.8、8.1、9.2 mmol L-1谷氨酰胺作用64 h。结果表明，谷氨酰胺可以促进鱼类肌细胞的增殖和分化。用含0，2.5。三点五四点五6.0 7.0 μ g mL（-1）维生素E处理96 h。结果表明，细胞增殖和分化没有明显增强，但抗超氧阴离子活性、抗羟自由基活性、还原型谷胱甘肽浓度有明显提高。经维生素E处理后，细胞内还原型谷胱甘肽与总谷胱甘肽的比值显著提高，培养液中丙二醛浓度显著降低。综合以上结果，本研究首次表明谷氨酰胺对鱼类肠上皮细胞的生长有促进作用，而维生素E对肠上皮细胞的生长无促进作用。维生素E能提高鱼类肠细胞的抗氧化能力和细胞结构的完整性。这些数据将对水产养殖饲料中谷氨酰胺和维生素E的补充具有指导意义。
The Present Study explored the effect Of glutamine and vitamin E oil growth and anti-oxidation capacity of isolated fish entrocytes. Fish enterocytes were Cultured with six medium, respectively, containing 0, 2.0, 4.7. 6.8, 8.1, 9.2 mmol L-1 glutamine for 64 h. The results showed that glutamine could promote fish entrocytes proliferation and differentiation. Fish enterocytes were cultured with different medium containing 0, 2.5. 3.5, 4.5. 6.0 7.0 mu g mL(-1) vitamin E for 96 h. The results showed that cells proliferation and differentiation were not significantly enhanced, but anti-superoxide anion activity, anti-hydroxy radical activity, reduced glutathione concentration. the ratio between reduced and total glutathione ill the cells were significantly enhanced, and the malondialdehyde concentration in the Culture medium was significantly depressed with the vitamin E treatment. In the whole, the present results firstly indicated that glutamine could promote fish enterocytes growth, but vitamin E Could not. Vitamin E could promote fish enterocytes antioxidant capacity and cellular structural integrity. These data would be instructive for glutamine and vitamin E supplement in aquaculture diets.