Hybrid polymeric hydrogels via peptide nucleic acid (PNA)/DNA complexation.
Hybrid polymeric hydrogels via peptide nucleic acid (PNA)/DNA complexation.
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DOI：
10.1016/j.jconrel.2015.09.035
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发表时间：
2015-12-28
期刊：
影响因子：
--
通讯作者：
Kopeček J
中科院分区：
文献类型：
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作者：
Chu TW;Feng J;Yang J;Kopeček J
这项工作提出了杂化水凝胶设计的一个新概念。接枝有多条肽核酸（PNA）的合成水溶性N -（2 - 羟丙基）甲基丙烯酰胺（HPMA）聚合物在加入连接DNA后发生交联。自组装是由PNA - DNA络合介导的，这导致了亲水性聚合物网络的形成。我们表明水凝胶可通过两种不同类型的络合来制备。I型水凝胶是通过PNA/DNA双螺旋杂交形成的。II型水凝胶利用了一种独特的“P型”寡核苷酸三螺旋结构，它由两条PNA序列和一条DNA组成。微流变学研究证实了各自的凝胶化过程，并表明与II型设计相比，I型凝胶具有更高的临界凝胶化浓度。扫描电子显微镜揭示了两种类型水凝胶相互连接的微孔结构。I型双螺旋水凝胶比II型三螺旋水凝胶具有更大的孔径。后者显然具有更致密的结构，并且也显示出更大的弹性。所设计的杂化水凝胶有作为新型生物材料用于制药和生物医学应用的潜力。
This work presents a new concept in hybrid hydrogel design. Synthetic water-soluble N-(2-hydroxypropyl)methacrylamide (HPMA) polymers grafted with multiple peptide nucleic acids (PNA) are crosslinked upon addition of the linker DNA. The self-assembly is mediated by the PNA–DNA complexation, which results in the formation of hydrophilic polymer networks. We show that the hydrogels can be produced through two different types of complexations. Type I hydrogel is formed via the PNA/DNA double-helix hybridization. Type II hydrogel utilizes a unique “P-form” oligonucleotide triple-helix that comprises two PNA sequences and one DNA. Microrheology studies confirm the respective gelation processes and disclose a higher critical gelation concentration for the type I gel when compared to the type II design. Scanning electron microscopy reveals the interconnected microporous structure of both types of hydrogels. Type I double-helix hydrogel exhibits larger pore sizes than type II triple-helix gel. The latter apparently contains denser structure and displays greater elasticity as well. The designed hybrid hydrogels have potential as novel biomaterials for pharmaceutical and biomedical applications.