PFGE and pertactin gene sequencing suggest limited genetic variability within the Finnish Bordetella parapertussis population.
PFGE and pertactin gene sequencing suggest limited genetic variability within the Finnish Bordetella parapertussis population.
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PFGE 和百日咳杆菌粘附素基因测序表明芬兰副百日咳博德特氏菌种群内的遗传变异有限。
DOI：
10.1099/jmm.0.05434-0
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发表时间：
2003
影响因子：
3
通讯作者：
Qiushui He
中科院分区：
文献类型：
--
作者：
J. Mäkinen;J. Mertsola;H. Soini;H. Arvilommi;M. Viljanen;N. Guiso;Qiushui He
百日咳杆菌、副百日咳杆菌和支气管败血杆菌的外膜蛋白（Prn）被认为是一种粘附素，是一种重要的免疫原。B的出现百日咳和B.支气管败血症Prn变体已被报道。本研究的目的是确定是否在B中发现类似的变异。副百日咳Prn和表征芬兰临床B。1982-2000年收集的副百日咳分离株。B 76号。在研究的副百日咳分离株中，发现7个（9%）具有沉默和非沉默核苷酸变化。此外，一个（1%）具有8个PQP重复而不是9个。三个密切相关的B。副百日咳菌群B.副百日咳是非常有限的，这表明B。副百日咳是一种稳定的生物体，能够很好地适应自己的生态位。
The outer-membrane protein pertactin (Prn) of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica is believed to function as an adhesin and is an important immunogen. The emergence of B. pertussis and B. bronchiseptica Prn variants has been reported. The aim of this study was to determine whether similar variation is found in B. parapertussis Prn and to characterize Finnish clinical B. parapertussis isolates that were collected in 1982-2000. Of 76 B. parapertussis isolates studied, seven (9 %) were found to have silent and non-silent nucleotide changes. In addition, one (1 %) had eight PQP repeats instead of nine. Three closely related B. parapertussis XbaI PFGE patterns were found. Genetic variation of B. parapertussis was found to be very limited, suggesting that B. parapertussis is a stable organism that is well-adapted to its own ecological niche.