The expression and activity of cystathionine-γ-lyase and 3-mercaptopyruvate sulfurtransferase in human neoplastic cell lines
The expression and activity of cystathionine-γ-lyase and 3-mercaptopyruvate sulfurtransferase in human neoplastic cell lines
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DOI：
10.1007/s00726-010-0606-3
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发表时间：
2011-06-01
期刊：
影响因子：
3.5
通讯作者：
Wrobel, Maria
中科院分区：
文献类型：
--
作者：
Jurkowska, Halina;Placha, Wojciech;Wrobel, Maria
研究了人肿瘤细胞系：星形细胞瘤U373、神经母细胞瘤SH-SY 5 Y、黑色素瘤A375和黑色素瘤WM 35中胱硫醚γ-裂解酶（CST）和3-巯基丙酮酸硫转移酶（MPST）的表达和活性。基因表达分析表明，所研究的肿瘤细胞显示MPST的表达，特别有趣的是，CST的表达。利用RT-PCR和Western印迹分析证实CST在这些细胞中的存在。然而，在U373细胞中，检测到非常低的CST活性。在所研究的所有细胞系中，MPST的活性高于CST，这表明在这些细胞中，硫烷硫形成的主要途径是MPST催化的反应。反相高效液相色谱分析表明，在所调查的肿瘤细胞中，胱硫醚和GSH的水平之间存在很大的差异。在SH-SY 5 Y细胞中，低水平的GSH和低GSH/GSSG比值对应着最高的CST活性。进一步的研究可能旨在验证CST在蛋白质或基因表达水平上的刺激是否可以改变肿瘤细胞的增殖。
The expression and activity of cystathionine gamma-lyase (CST) and 3-mercaptopyruvate sulfurtransferase (MPST) were investigated in the human neoplastic cells lines: astrocytoma U373, neuroblastoma SH-SY5Y, melanoma A375, and melanoma WM35. Gene expression analysis demonstrated that the investigated neoplastic cells showed the expression of MPST and what is particularly interesting, the expression of CST. The presence of CST in these cells was confirmed using RT-PCR and western blot analysis. However, in U373 cells, a very low activity of CST was detected. In all the investigated cell lines, the activity of MPST was higher than that of CST, which suggests that in these cells, the main pathway of sulfane sulfur formation is the MPST-catalyzed reaction. RP-HPLC analysis showed a large disparity between the level of cystathionine and GSH in the investigated neoplastic cells. In SH-SY5Y cells, the low level of GSH and low GSH/GSSG ratio corresponded with the highest CST activity. Further investigations could aim at verifying whether the stimulation of CST, at the level of protein or gene expression, could change the proliferation of neoplastic cells.