A 24,482-bp deletion is associated with increased seed weight in Brassica napus L.
A 24,482-bp deletion is associated with increased seed weight in Brassica napus L.
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DOI：
10.1007/s00122-021-03850-x
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发表时间：
2021-05-17
影响因子：
5.4
通讯作者：
Hong, Dengfeng
中科院分区：
文献类型：
--
作者：
Zhang, Xiaohui;Huang, Qiyang;Hong, Dengfeng
油菜种子质量的一个主要QTL被精细定位，一个24482 bp的缺失可能通过多种途径介导了这一效应。探索控制种子重的基因对提高作物产量和阐明油菜种子形成机制具有重要意义。我们之前鉴定了数量性状位点qSW。以亲本材料HZ396和Y106的F-1杂种构建的双单倍体群体的千粒重（TSW）为C9。在这里，我们证实了与qSW相关的表型效应。在BC3F2人群中发现C9，并将候选因果位点精确定位到266 kb的区间。序列和表达分析显示，HZ396中包含6个预测基因的24,482 bp缺失最有可能是qSW.C9的基础。差异基因表达分析和细胞学观察提示qSW。C9通过多种信号通路影响细胞增殖和细胞扩增。通过对菜籽多样性面板进行基因分型，确定单倍型结构后，可以得出结论，具有两个特异性标记的种质选择可能有效地提高了菜籽的种子质量。本研究为qSW致病基因的鉴定奠定了坚实的基础。为高产油菜籽的选育提供了一个有希望的靶点。
Key message A major QTL for seed weight was fine-mapped in rapeseed, and a 24,482-bp deletion likely mediates the effect through multiple pathways. Exploration of the genes controlling seed weight is critical to the improvement of crop yield and elucidation of the mechanisms underlying seed formation in rapeseed (Brassica napus L.). We previously identified the quantitative trait locus (QTL) qSW.C9 for the thousand-seed weight (TSW) in a double haploid population constructed from F-1 hybrids between the parental accessions HZ396 and Y106. Here, we confirmed the phenotypic effects associated with qSW.C9 in BC3F2 populations and fine-mapped the candidate causal locus to a 266-kb interval. Sequence and expression analyses revealed that a 24,482-bp deletion in HZ396 containing six predicted genes most likely underlies qSW.C9. Differential gene expression analysis and cytological observations suggested that qSW.C9 affects both cell proliferation and cell expansion through multiple signaling pathways. After genotyping of a rapeseed diversity panel to define the haplotype structure, it could be concluded that the selection of germplasm with two specific markers may be effective in improving the seed weight of rapeseed. This study provides a solid foundation for the identification of the causal gene of qSW.C9 and offers a promising target for the breeding of higher-yielding rapeseed.