Determination of chlorophylls and carotenoids of marine phytoplankton: separation of chlorophyll a from divinylchlorophyll a and zeaxanthin from lutein
Determination of chlorophylls and carotenoids of marine phytoplankton: separation of chlorophyll a from divinylchlorophyll a and zeaxanthin from lutein
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DOI：
10.1093/plankt/18.12.2377
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发表时间：
1996-12
影响因子：
2.1
通讯作者：
F. Vidussi;H. Claustre;J. Bustillos-Guzmán;C. Cailliau;J. Marty
中科院分区：
文献类型：
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作者：
F. Vidussi;H. Claustre;J. Bustillos-Guzmán;C. Cailliau;J. Marty
本文提出了一种快速的反相高效液相色谱法，用于浮游植物色素的鉴定和定量。该方法对二乙烯基叶绿素a和叶绿素b进行了拆分，对叶黄素和玉米黄质以及二乙烯基叶绿素b进行了部分拆分。此外，叶绿素c 1、c 2和c 3也得到了很好的分离。一个样品的分析时间为20 mm，这使得该方法特别适合需要处理大量样品的情况。
A rapid reverse-phase HPLC method is presented for the identification and quantification of most of the phytoplankton pigments. This method yields the resolution of divinyl-chlorophyllaand chlorophylla, as well as the partial resolution of lutein and zeaxanthin, and of divinyl-chlorophyllband chlorophyllb. In addition, chlorophyllsc1,2andc3are well resolved. The analysis time for one sample is 20 mm, which makes this method particularly suited when large numbers of samples have to be processed.