A reductase gene mppE controls yellow component production in azaphilone polyketide pathway of Monascus
A reductase gene mppE controls yellow component production in azaphilone polyketide pathway of Monascus
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DOI：
10.1007/s10529-016-2232-y
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发表时间：
2017-01-01
影响因子：
2.7
通讯作者：
Kwon, Hyung-Jin
中科院分区：
文献类型：
--
作者：
Balakrishnan, Bijinu;Park, Si-Hyung;Kwon, Hyung-Jin
为了鉴定红曲霉中选择性参与氮杂菲酮聚酮途径中黄色或橙子组分生物合成的基因，预测红曲霉氮杂菲酮色素生物合成途径中还原（黄色）和氧化（橙子和红色）组分的相对水平受还原修饰的控制。还原酶基因mppE的靶向失活增强了橙子和红色色素的产生，而该基因的过表达促进了黄色色素的产生。mppE过表达的影响取决于培养方法，并且在使用化学成分确定的培养基的深层培养中，黄色色素沉着明显增加。MppE作为阿扎菲隆聚酮途径中的还原酶，控制黄色素、安卡黄素和红曲霉素的生物合成。
To characterize a biosynthetic gene that is selectively involved in the biosynthesis of yellow or orange components in the azaphilone polyketide pathway of Monascus.A reductive modification is predicted to control the relative levels of reduced (yellow) and oxidized (orange and red) components in the pathway of azaphilone pigment biosynthesis in Monascus. Targeted inactivation of a reductase gene mppE enhanced orange and red pigment production whereas overexpression of the gene promoted yellow pigment production. The effect of mppE overexpression was dependent on culture methods, and augmented yellow pigmentation was evident in a submerged culture employing a chemically defined medium.MppE controls the biosynthesis of the yellow pigments, ankaflavin and monascin, as a reductive enzyme in the azaphilone polyketide pathway.