LOCALIZATION OF PROTEIN PROTEIN INTERACTIONS BETWEEN SUBUNITS OF PHYTOCHROME
LOCALIZATION OF PROTEIN PROTEIN INTERACTIONS BETWEEN SUBUNITS OF PHYTOCHROME
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DOI：
10.1105/tpc.4.2.161
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发表时间：
1992-02-01
期刊：
影响因子：
11.6
通讯作者：
JONES, AM
中科院分区：
文献类型：
--
作者：
EDGERTON, MD;JONES, AM
我们使用了一种基于蛋白质与lambda-阻遏因子融合的新方法来鉴定光敏色素羧基末端区域内能够介导二聚化的两个小区域。利用体内实验，DNA结合、lambda-阻遏物氨基末端结构域和燕麦PhyA光敏色素片段之间的融合检测了阻遏物活性的增加，这是二聚化的一个指标。在这个分析系统中，燕麦光敏色素在氨基酸V623-S673和N1049-Q1129之间的区域显示出增加抑制活性。这些短跨度在属于光敏色素PhyA家族的蛋白质之间高度保守。在这些序列中嵌入了四个片段，它们可能形成两相性的α螺旋。其中两个片段在PhyA光敏色素和phyB和phyC基因编码的光敏色素之间保守，表明异源二聚体可能是通过亚基相互作用在这些位点形成的。
We have used a novel assay based on protein fusions with lambda-repressor to identify two small regions within phytochrome's carboxy-terminal domain that are capable of mediating dimerization. Using an in vivo assay, fusions between the DNA binding, amino-terminal domain of lambda-repressor and fragments from oat PhyA phytochrome have been assayed for increased repressor activity, an indicator of dimerization. In this assay system, regions of oat phytochrome between amino acids V623-S673 and N1049-Q1129 have been shown to increase repressor activity. These short spans are highly conserved between proteins belonging to the phytochrome PhyA family. Embedded within these sequences are four segments that could potentially form amphipathic alpha helices. Two of the segments are well conserved between PhyA phytochrome and phytochromes encoded by the phyB and phyC genes, suggesting that heterodimers might form by way of subunit interaction at these sites.