Propolis analysis

Propolis analysis
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DOI:
10.1590/s0101-20612006000100028
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发表时间:
2006-03-01
期刊:
Food Science and Technology (Campinas)
影响因子:
--
通讯作者:
Ferro, Vicente O.
Ferro, Vicente O.
中科院分区:
其他
文献类型:
--
作者:
Funari, Cristiano S.;Ferro, Vicente O.

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这项工作的目标是有助于建立常规的质量控制,包括其植物来源的识别。为此,我们根据巴西农业部的建议(感官评价、105°C干燥失重、可提取和不可提取物质的测定以及灰分、蜡、黄酮类化合物和总酚含量的测定)和比较色谱分析(HPLC),对可可豆及其预期的植物来源进行了分析。除“105°C下的干燥失重测试”外,所有参数均在农业部规定的限度内,以保证玉米的特性和质量。我们的研究结果表明,种Baccharis dracunculifolia DC,是从塞拉做日本(圣保罗州)/巴西的蔬菜来源。可以鉴定出蜂胶和植物中的酸类化合物:阿替匹林C、副肉桂酸、阿魏酸、绿原酸、咖啡酸和反式西马酸,以及黄酮类化合物:山奈素和异樱花素。
The goal of this work is to contribute in establishing routines for the quality control of propolis, including the identification of its botanical source(s). For this reason, we present an analysis in accordance with the Brazilian Agricultural Ministry recommendations (organoleptic evaluation, loss on drying at 105°C, determinations of extractable and non-extractable matter and determinations of ash, wax, flavonoids and total phenolic contents) and comparative chromatographic analysis (HPLC) between propolis and its expected vegetal source. With the exception of the "drying loss test at 105°C", all the parameters were within the limits established by the Ministry of Agricultural to guarantee the identity and quality of propolis. Our results suggested that the species Baccharis dracunculifolia DC, was the vegetable source of the propolis from Serra do Japi (Sao Paulo State)/Brazil. Identification of the acids artepillin C, para-cournaric, ferulic, chlorogenic, caffeic and trans-cirmamic and of the flavonoids kaempferide and isosakuranetin in propolis and plant was possible.