Calcium channel currents recorded from isolated myocytes of rat basilar artery are stretch sensitive.
Calcium channel currents recorded from isolated myocytes of rat basilar artery are stretch sensitive.
复制标题
从大鼠基底动脉分离的肌细胞记录的钙通道电流是拉伸敏感的。
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
P. Langton
中科院分区：
文献类型：
--
作者：
P. Langton
1.使用常规和穿孔膜片全细胞方法测量离体大鼠基底动脉肌细胞响应电压阶跃的Ca 2+通道电流。在获得全细胞接入后的前3分钟内，响应于重复步进至+2 mV而测量的峰值电流增加。增加遵循指数时间过程，是不是由于减少潜在的外向电流，是独立的存在下的ATP和GTP的移液管填充解决方案。2.施加正压和负压的移液管导致显着增加和减少的峰值内向电流，分别。膜拉伸，与细胞体积的变化，而不是施加的压力本身出现，以确定峰值电流的变化。3.使用白藜芦醇B穿孔膜片记录，发现应用80%低渗或120%高渗灌注溶液时细胞体积的变化对内向电流峰值的影响相似。低渗引起的细胞肿胀增加和高渗细胞收缩降低峰值内向电流在所有测试电位研究。4.响应低渗灌注液的内向电流增加是可逆的，似乎反映了电压依赖性Ca 2+通道电流的增加，因为它不是由于非电压敏感性电导的变化，并且它与对照电流一样对二氢吡啶（DHP）拮抗剂（-）202 - 791敏感。
1. Conventional and perforated patch whole‐cell methods were used to measure Ca2+ channel currents from isolated rat basilar arterial myocytes in response to voltage steps. Peak currents measured in response to repeated steps to +2 mV increased over the first 3 min after gaining whole‐cell access. The increase followed an exponential time course, was not due to decrease in underlying outward current and was independent of the presence of ATP and GTP in the pipette filling solution. 2. Application of positive and negative pressure to the pipette resulted in significant increase and decrease in peak inward current, respectively. Membrane stretch, associated with changes in cell volume, rather than applied pressure per se appeared to determine changes in peak current. 3. Using amphotericin B perforated patch recording, changes in cell volume on application of 80% hypo‐ or 120% hyperosmotic superfusing solutions were found to effect similarly peak inward current. Hyposmotically induced cell swelling increased and hyperosmotic cell shrinkage decreased peak inward current at all test potentials studied. 4. The increase in inward current in response to hyposmotic superfusate was reversible and appears to reflect an increase in voltage‐dependent Ca2+ channel current since it was not due to a change in non‐voltage‐sensitive conductance(s) and it was as sensitive as the control current to the dihydropyridine (DHP) antagonist (‐)202‐791.
通过电压、血清素和二氢吡啶调节脑动脉中的单个钙通道。
DOI：
10.1152/ajpheart.1991.261.6.h1951
发表时间：
1991
期刊：
The American journal of physiology
影响因子：
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作者：
Worley,JF;Quayle,JM;Standen,NB;Nelson,MT
通讯作者：
Nelson,MT
影响因子：
3.4
作者：
SOKABE, M;SACHS, F;JING, ZQ
通讯作者：
JING, ZQ