Synthetic organic pH buffers can support fertilization of guinea pig eggs, but not as efficiently as bicarbonate buffer.
Synthetic organic pH buffers can support fertilization of guinea pig eggs, but not as efficiently as bicarbonate buffer.
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合成有机 pH 缓冲剂可以支持豚鼠卵受精，但不如碳酸氢盐缓冲剂有效。
DOI：
10.1002/mrd.1120190203
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Yanagimachi,R
中科院分区：
文献类型：
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作者：
Bhattacharyya,A;Yanagimachi,R
培养基中的碳酸氢根离子（HCO3−）对豚鼠卵的受精并不是绝对必要的。然而，受精在HCO3−缓冲培养基中最有效。精子的获能、顶体反应和精子穿透可以在含有合成有机缓冲液的无HCO3−培养基中发生（即，MOPS、TES、HEPES、Tris和TAPSO），但不如在HCO3−缓冲培养基中有效。HCO3−的功能似乎不仅仅是pH缓冲分子。
Bicarbonate ions (HCO3−) in the medium are not absolutely essential for the fertilization of guinea pig eggs. However, fertilization takes place most efficiently in HCO3−‐buffered medium. Capacitation, the acrosome reaction, and zona penetration by spermatozoa can occur in HCO3−‐free media with synthetic organic buffers (i.e., MOPS, TES, HEPES, Tris, and TAPSO) but not as efficiently as in the HCO3−‐buffered medium. It appears that HCO3−functions as more than just a pH‐buffering molecule.