Physiological, biochemical and proteomic responses of rice ( Oryza sativa L.)varieties Godaheenati and Pokkali for drought stress at the seedling stage
Physiological, biochemical and proteomic responses of rice ( Oryza sativa L.)varieties Godaheenati and Pokkali for drought stress at the seedling stage
复制标题
水稻品种 Godaheenati 和 Pokkali 对苗期干旱胁迫的生理、生化和蛋白质组反应
DOI：
10.4038/tar.v27i2.8164
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发表时间：
2016
影响因子：
--
通讯作者：
H. A. M. Wickramasinghe
中科院分区：
文献类型：
--
作者：
J. K. P. T. P. Jayaweera;H. M. V. G. Herath;D. Jayatilake;G. S. Udumulla;H. A. M. Wickramasinghe
非生物胁迫是限制水稻生产的关键限制因素。其中，干旱是诱发植物多种生理、生化和分子反应的主要因素之一。筛选抗旱水稻品种是培育抗旱水稻品种的先决条件。根据苗期生化属性筛选了两个水稻品种 Godaheenati (4049) 和 Pokkali 的干旱胁迫反应。在五天的干旱期间测量了相对含水量、两周大稻苗叶片的总蛋白质含量和光合色素水平。测试的品种具有不同的相对含水量、蛋白质浓度和色素水平，这可能是由于它们的遗传差异。 Godaheenati在干旱胁迫下第四天表现出相对含水量的增加，表明其细胞恢复能力，因此可能比Pokkali更耐旱。脱水胁迫导致两个品种中所有三种光合色素的波动。第四天，Godaheenati的光合色素比Pokkali显着增加，这可能是由于水分含量增加而导致植物细胞恢复的结果。从暴露于干旱条件下的两周龄Godaheenati水稻幼苗叶片中提取的总蛋白含量进行二维凝胶电泳。结果显示水稻叶片总蛋白质组发生改变，并且水稻耐旱基因上调。建议对 Godaheenati 作为耐旱水稻品种进行进一步筛选，用于水稻育种计划。
Abiotic stresses are key limiting factors that restrict rice production. Among them, drought is one of the major factors that induce several physiological, biochemical and molecular responses in plants. Screening of rice varieties against drought stress is a prerequisite to produce stress tolerant rice varieties. Two rice varieties, i.e. Godaheenati (4049) and Pokkali were screened for drought stress responses based on biochemical attributes at the seedling stage. Relative water content, the total protein content of leaves of two-week-old rice seedlings and photosynthetic pigment levels were measured over a fiveday drought period. The varieties tested responded with different relative water content, protein concentrations and pigment levels, potentially due to their genetic differences. Godaheenati showed an increase of the relative water content on the fourth day under drought stress, indicative of its cell recovering capability, and thus may be drought tolerant than Pokkali . Dehydration stress resulted in the fluctuation of all three photosynthetic pigments in both varieties. On the fourth day, Godaheenati showed a significant increase of photosynthetic pigments than Pokkali , and this could be a result of recovery of plant cells due to the increase of water content. The total protein content extracted from leaves of twoweek- old rice seedlings of Godaheenati exposed to drought conditions was subjected to twodimensional gel electrophoresis.The results showed alterations in the total proteome of rice leaves, and an up regulation of genes responsible for drought stress tolerance in rice. Further screening is recommended for Godaheenati as a drought tolerant rice variety to be used in rice breeding programs.