Blood group A and B antigens are strongly expressed on platelets of some individuals
Blood group A and B antigens are strongly expressed on platelets of some individuals
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DOI：
10.1182/blood.v96.4.1574.h8001574_1574_1581
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发表时间：
2000-08-15
期刊：
影响因子：
20.3
通讯作者：
Aster, RH
中科院分区：
文献类型：
--
作者：
Curtis, BR;Edwards, JT;Aster, RH
人们普遍认为，血小板上 ABH 抗原的表达不足以对输血受者中 ABH 不相容血小板的存活产生重大影响，但 A 和 B 不相容血小板存活率较差的轶事报道表明情况并非总是如此。对100名A组（1）和B组献血者的血小板上A和B抗原的表达进行了测量，分别有7%和4%的血小板A和B抗原水平持续超过平均值加2SD。在流式细胞术和统计分析的基础上，将血小板中A抗原含量高于正常水平的献血者细分为2组，称为I型和II型（“高表达者”）。 A组和B组高表达者血清A(1)-和B-糖基转移酶水平显着高于A(1)组和B组正常表达个体。高表达者的红细胞上的 H 抗原水平较低，表明该异常影响其他细胞谱系。免疫化学研究表明，高表达者血小板的各种糖蛋白 (GP) 上的 A 抗原水平较高，尤其是 GPIIb 和 PECAM (CD31)。 A(1) II 型高表达者表型在一个家族中作为常染色体显性性状遗传。一个 II 型 A(1) 高表达者的 A(1) 转移酶基因的外显子 5、6 和 7 的序列以及来自其他 3 个基因的外显子 7 的序列与报道的正常序列相同。需要进一步研究来定义高表达者性状的分子基础并表征其临床意义（Blood，2000；96：1574-1581）（C）2000，美国学会血液学。
It is widely thought that expression of ABH antigens on platelets is insufficient to materially affect the survival of ABH-incompatible platelets in transfusion recipients, but anecdotal reports of poor survival of A and B mismatched platelets suggest that this is not always the case. The A and B antigen expression on platelets of 100 group A(1) and group B blood donors was measured, and 7% and 4%, respectively, had platelets whose A and B antigen levels consistently exceeded the mean plus 2 SD, On the basis of flow cytometric and statistical analysis, donors whose platelets contained higher than normal levels of A antigen were subdivided into 2 groups, designated Type I and Type II ("high expressers"). Serum A(1)- and B-glycosyltransferase levels of A and B high expressers were significantly higher than those of group A(1) and B individuals with normal expression. H antigen levels were low on the red cells of high expressers, indicating that the anomaly affects other cell lineages, Immunochemical studies demonstrated high levels of A antigen on various glycoproteins (GPs) from high-expresser platelets, especially GPIIb and PECAM (CD31). The A(1) Type II high-expresser phenotype was inherited as an autosomal dominant trait in one family. The sequences of exons 5, 6, and 7 of the A(1)-transferase gene of one Type II A(1) high expresser and exon 7 from 3 other genes were identical to the reported normal sequences, Further studies are needed to define the molecular basis for the high-expresser trait and to characterize its clinical implications, (Blood, 2000;96:1574-1581) (C) 2000 by The American Society of Hematology.