Spatial genetic structure within a metallicolous population of Arabidopsis halleri, a clonal, self‐incompatible and heavy‐metal‐tolerant species
Spatial genetic structure within a metallicolous population of Arabidopsis halleri, a clonal, self‐incompatible and heavy‐metal‐tolerant species
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DOI：
10.1111/j.1365-294x.2004.02314.x
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发表时间：
2004-10
影响因子：
4.9
通讯作者：
F. Van Rossum;I. Bonnin;Stéphane Fénart;M. Pauwels;D. Petit;P. Saumitou-Laprade
中科院分区：
文献类型：
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作者：
F. Van Rossum;I. Bonnin;Stéphane Fénart;M. Pauwels;D. Petit;P. Saumitou-Laprade
拟南芥（Arabidopsis halleri）是A.拟南芥是一种耐重金属（Zn、Pd、Cd）的克隆、昆虫授粉草本植物，并且是Zn和Cd的超富集植物。它是特别感兴趣的进化过程和植物修复的研究。然而，很少有人知道它的种群基因流模式和遗传多样性的结构。利用5个微卫星位点在10 cm ~ 500 m的空间尺度上研究了金斑菊的遗传结构。halleri。我们还研究了克隆繁殖和有性繁殖（种子和花粉传播）的遗传模式的贡献。克隆多样性较高（DG > 0.9）。克隆传播仅发生在短距离（< 1米）。克隆扩散和有限扩散，与有性繁殖，有助于显着的空间遗传结构揭示的空间自相关分析。自相关图的形状表明，种子传播受到限制，花粉流广泛，这可能与昆虫传粉者的强烈活动有关。低污染区的克隆扩散比高污染区更广泛。这不能被解释为一种策略，以促进最苛刻的生态约束下（最高的重金属浓度）的适应基因型的繁殖。在低污染区发现的更高的精细尺度空间遗传结构可以归因于植物密度低于高污染区。没有证据表明，遗传分化，由于空间重金属异质性之间的低，高污染区。
Arabidopsis halleri, a close wild relative of A. thaliana, is a clonal, insect‐pollinated herb tolerant to heavy metals (Zn, Pd, Cd) and a hyperaccumulator of Zn and Cd. It is of particular interest in the study of evolutionary processes and phytoremediation. However, little is known about its population gene flow patterns and the structure of its genetic diversity. We used five microsatellite loci to investigate the genetic structure at a fine spatial scale (10 cm to 500 m) in a metallicolous population of A. halleri. We also studied the contributions made by clonal propagation and sexual reproduction (seed and pollen dispersal) to the genetic patterns. Clonal diversity was high (DG > 0.9). Clonal spread occurs only at short distances (< 1 m). Both clonal spread and limited dispersal, associated with sexual reproduction, contribute to the significant spatial genetic structure revealed by spatial autocorrelation analysis. The shape of the autocorrelogram suggests that seed dispersal is restricted and pollen flow extensive, which may be related to intense activity by insect pollinators. Clonal spread was more extensive in the lowly polluted zone than in the highly polluted zone. This cannot be interpreted as a strategy for promoting the propagation of adapted genotypes under the harshest ecological constraints (highest heavy metal concentrations). The higher fine‐scale spatial genetic structure found in the lowly polluted zone can be ascribed to plant densities that were lower than in the highly polluted zone. No evidence of genetic divergence due to spatial heavy metal heterogeneity was found between lowly and highly polluted zones.