Nasopharyngeal Colonization by Potentially Pathogenic Bacteria Found in Healthy Semi-Captive Wild-Born Chimpanzees in Uganda
Nasopharyngeal Colonization by Potentially Pathogenic Bacteria Found in Healthy Semi-Captive Wild-Born Chimpanzees in Uganda
复制标题
DOI：
10.1002/ajp.22212
复制
发表时间：
2014-02-01
影响因子：
2.4
通讯作者：
Leendertz, Fabian H.
中科院分区：
文献类型：
--
作者：
Mugisha, Lawrence;Koendgen, Sophie;Leendertz, Fabian H.
关于黑猩猩在圈养保护区和野外定居的信息很少见。本研究旨在建立保护区黑猩猩中的鼻疽定植和潜在细菌病原体，作为改善黑猩猩和员工健康的基础。采用微生物培养法和针对细菌16 SrRNA基因的聚合酶链反应（PCR）技术，对39只健康黑猩猩的鼻咽炎菌定植情况进行了分析。我们报告了四个主要门占主导地位的变形菌门（50%），Fermicutes（35.7%），拟杆菌（7.1%），和Cynobacteria（7.1%）在健康的半圈养黑猩猩。基于7-碱基寡聚体的进一步分类揭示了以下属：链球菌属、韦荣氏球菌属、奈瑟氏球菌属、普雷沃氏菌属、金氏菌属和未分类的食蟹猴杆菌属、放线杆菌属、拟杆菌属和巴斯德氏菌科。在微生物培养上，我们能够鉴定和表征一些细菌的种属水平，如肺炎克雷伯氏菌和铜绿假单胞菌是优势细菌，分别具有54.7%和50%的定植。其中，链球菌属、奈瑟氏菌属、克雷伯氏菌属和嗜血杆菌属具有已知潜在引起严重呼吸道疾病的代表。我们的数据提供了关于黑猩猩nasophyridium殖民的重要信息，作为了解改善黑猩猩健康所需的疾病过程和药物治疗的指南。这项研究的结果将指导改善保护区黑猩猩日常管理程序的过程，并将其作为评估未来重新引入可能性的基础。Am. J. Primatol. 76：103-110，2014. (c)2013 Wiley Periodicals，Inc.
Information on the chimpanzee nasopharygeal colonization in captive sanctuaries and in the wild is rare. This study was undertaken to establish the nasopharygeal colonization and potential bacterial pathogens in sanctuary chimpanzees as a basis for improving chimpanzee and employee health. Nasopharygeal colonization of 39 healthy chimpanzees were analyzed by microbiological cultivation method and polymerase chain reaction (PCR) targeting the bacterial 16S rRNA gene. We report four major phyla dominated by Proteobacteria (50%), Fermicutes (35.7%), Bacteriodes (7.1%), and Cynobacteria (7.1%) in healthy semi-captive chimpanzees. Further classification based on 7-base oligomers revealed the following genera: Streptococcus, Veillonella, Neisseria, Prevotella, Kingella and unclassified Cynobacteria, Actinobacillus, Bacteriodes and Pasteurellaceae. On microbiological cultivation we were able to identify and characterize some of the bacteria to species level as Klebsiella pneumonie and Pseudomonas aeruginosa being dominant bacteria with 54.7% and 50% colonization, respectively. Of these, Streptococcus, Neisseria, Klebsiella, and Haemophillus have representatives known to potentially cause severe respiratory disease. Our data present important information on chimpanzee nasopharygeal colonization as a guide to understanding disease processes and pharmaceutical therapies required for improving the health of chimpanzees. The results from this study will guide the processes to improve procedures for routine management of sanctuary chimpanzees and use it as a basis for evaluation of future reintroduction possibilities. Am. J. Primatol. 76:103-110, 2014. (c) 2013 Wiley Periodicals, Inc.