Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration.
Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration.
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DOI：
10.3390/ijms24010466
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发表时间：
2022-12-27
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
由于外切体能够促进细胞间的通讯，从而增强细胞募集、谱系特异性分化和组织再生，因此引起了人们的关注。本研究的目的是确定外切体对牙髓再生过程中细胞归巢和血管生成分化的影响。从生长(Exo-G)或血管生成分化(Exo-A)条件下培养的兔牙髓干细胞中分离出外切体(DPSC-Exos)。通过纳米颗粒跟踪分析和抗体阵列证实了外切体的特征。5×108/mLExosome处理后，DPSC-Exos可显著促进细胞增殖和迁移。在基因表达分析中，DPSC-Exos增强了血管生成标志物的表达，包括血管内皮生长因子A(VEGFA)、FMS相关酪氨酸激酶1(Flt1)和血小板与内皮细胞黏附分子1(PECAM1)。此外，我们在Exo-A中确定了关键的外体microRNAs，用于细胞归巢和血管生成。综上所述，基于外周小体的细胞归巢和血管生成分化策略在牙髓再生方面具有显著的治疗潜力。
Exosomes have attracted attention due to their ability to promote intercellular communication leading to enhanced cell recruitment, lineage-specific differentiation, and tissue regeneration. The object of this study was to determine the effect of exosomes on cell homing and angiogenic differentiation for pulp regeneration. Exosomes (DPSC-Exos) were isolated from rabbit dental pulp stem cells cultured under a growth (Exo-G) or angiogenic differentiation (Exo-A) condition. The characterization of exosomes was confirmed by nanoparticle tracking analysis and an antibody array. DPSC-Exos significantly promoted cell proliferation and migration when treated with 5 × 108/mL exosomes. In gene expression analysis, DPSC-Exos enhanced the expression of angiogenic markers including vascular endothelial growth factor A (VEGFA), Fms-related tyrosine kinase 1 (FLT1), and platelet and endothelial cell adhesion molecule 1 (PECAM1). Moreover, we identified key exosomal microRNAs in Exo-A for cell homing and angiogenesis. In conclusion, the exosome-based cell homing and angiogenic differentiation strategy has significant therapeutic potential for pulp regeneration.
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影响因子：
11.2
作者：
Bader AG;Brown D;Winkler M
通讯作者：
Winkler M
影响因子：
3.7
作者：
Bronckaers A;Hilkens P;Fanton Y;Struys T;Gervois P;Politis C;Martens W;Lambrichts I
通讯作者：
Lambrichts I
影响因子：
4
作者：
Hakulinen, Juha;Sankkila, Lotta;Keski-Oja, Jorma
通讯作者：
Keski-Oja, Jorma
影响因子：
3.9
作者：
Ivica, Anja;Ghayor, Chafik;Weber, Franz E.
通讯作者：
Weber, Franz E.
影响因子：
4.4
作者：
Jimenez, Lizandra;Yu, Hui;Weaver, Alissa M.
通讯作者：
Weaver, Alissa M.