Identification and characterization of maize ACD6-like gene reveal ZmACD6 as the maize orthologue conferring resistance to Ustilago maydis.
Identification and characterization of maize ACD6-like gene reveal ZmACD6 as the maize orthologue conferring resistance to Ustilago maydis.
复制标题
玉米 ACD6 样基因的鉴定和表征表明 ZmACD6 是赋予玉米黑粉菌抗性的玉米直系同源物。
DOI：
10.1080/15592324.2019.1651604
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发表时间：
2019
影响因子：
2.9
通讯作者：
Jingtang Chen
中科院分区：
文献类型：
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作者：
Zhongqin Zhang;Jinjie Guo;Yongfeng Zhao;Jingtang Chen
增强广谱抗性是作物育种的一个主要目标。然而，广谱耐药性尚未得到彻底研究，其潜在的分子机制仍然难以捉摸。在模式植物拟南芥 (Arabidopsis thaliana) 中，加速细胞死亡6 (ACD6) 是广谱抗性的关键组成部分，与水杨酸 (SA) 形成正反馈回路，调节多种模式识别受体。然而，ACD6 在作物抗病性中的作用尚不清楚。在这里，我们发现ANK23（玉米（Zea mays）中15个ACD6样基因之一）的转录物是由SA和病原真菌玉米黑粉菌（Ustilago maydis）感染诱导的。ANK23的异源表达恢复了拟南芥突变体acd6-2的抗病性。我们证明 ANK23 是 ACD6 的玉米直系同源物，因此将 ANK23 重命名为 ZmACD6。此外，使用 CRISPR/Cas9，我们生成了 ZmACD6 敲除玉米植物，这些植物更容易受到 U.玉米比野生型植物。我们还从不同的天然玉米群体中鉴定出具有相对较高 ZmACD6. 表达水平的玉米品系 (SC-9)。 SC-9 增强了对 U 的抗病性。玉米和防御激活，提出了培育具有增强抗病性的优良品种的实用方法。
Enhancing broad-spectrum resistance is a major goal of crop breeding. However, broad-spectrum.resistance has not been thoroughly investigated, and its underlying molecular mechanisms remain.elusive. In the model plant Arabidopsis (Arabidopsis thaliana), ACCELERATED CELL DEATH6 (ACD6) is.a key component of broad-spectrum resistance that acts in a positive feedback loop with salicylic acid.(SA) to regulate multiple pattern recognition receptors. However, the role of ACD6 in disease resistance.in crop plants is unclear. Here, we show that the transcript of ANK23, one of the 15 ACD6-like genes in.maize (Zea mays), is induced by SA and by infection with the pathogenic fungus Ustilago maydis..Heterologous expression of ANK23 restored disease resistance in the Arabidopsis mutant acd6-2. We.show that ANK23 is a maize ortholog of ACD6 and therefore rename ANK23 as ZmACD6. Furthermore,.using CRISPR/Cas9, we generated ZmACD6 knockout maize plants, which are more susceptible to.U. maydis than wild-type plants. We also identified a maize line (SC-9) with relatively high ZmACD6.expression levels from a diverse natural maize population. SC-9 has increased disease resistance to.U. maydis and defense activation, suggesting a practical approach to cultivate elite varieties with.enhanced disease resistance.