RETINAL-PIGMENT EPITHELIAL-CELLS IN EPIRETINAL MEMBRANES - AN IMMUNOHISTOCHEMICAL STUDY
RETINAL-PIGMENT EPITHELIAL-CELLS IN EPIRETINAL MEMBRANES - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1136/bjo.68.10.708
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发表时间：
1984-01-01
影响因子：
4.1
通讯作者：
MCLEOD, D
中科院分区：
文献类型：
--
作者：
HISCOTT, PS;GRIERSON, I;MCLEOD, D
使用免疫组织化学技术来鉴定来自眼部（参考）组织的切片或组织培养单层中含有细胞角蛋白的细胞，以及在针对黄斑皱褶或大规模视网膜前回缩的闭合显微手术期间获得的 22 个视网膜前膜中。参考组织中细胞角蛋白免疫染色的结果表明，这是确定视网膜前膜中上皮细胞的贡献和分布的有价值的手段，并且膜中的上皮细胞可能源自视网膜色素上皮。在 22 个视网膜前膜中的 17 个中鉴定出上皮细胞，但它们通常不构成主要细胞类型。显然，被认为负责视网膜前膜收缩的成纤维细胞或成纤维细胞样细胞很少源自视网膜色素上皮。膜的生物显微镜色素沉着对其上皮细胞群的指导很差。
Immunohistochemical techniques were used to identify cells containing cytokeratins in sections or tissue-culture monolayers from ocular (reference) tissues and also from 22 epiretinal membranes obtained during closed microsurgery for macular pucker or massive preretinal retraction. Results of cytokeratin immunostaining in reference tissues indicated that this is a valuable means of determining the contribution and distribution of epithelial cells in epiretinal membranes, and that the epithelial cells in the membranes were probably derived from the retinal pigment epithelium. Epithelial cells were identified in 17 of the 22 epiretinal membranes, but they did not usually constitute the predominant cell type. Apparently, the fibroblasts or fibroblast-like cells thought to be responsible for the contraction of epiretinal membranes are seldom of retinal pigment epithelial origin. Biomicroscopic pigmentation of a membrane was a poor guide to its epithelial cell population.