Endosomal recycling controls plasma membrane area during mitosis
Endosomal recycling controls plasma membrane area during mitosis
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DOI：
10.1073/pnas.0702511104
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发表时间：
2007-05-08
影响因子：
11.1
通讯作者：
Kirchhausen, Tomas
中科院分区：
文献类型：
--
作者：
Boucrot, Emmanuel;Kirchhausen, Tomas
细胞及其子细胞的形状和总表面在有丝分裂期间发生变化。许多细胞在前期和中期聚集，并在胞质分裂期间重新获得其延伸和扁平的形状。质膜的总面积如何变化以适应这些形态变化以及通过什么机制实现总膜面积的控制？使用单细胞成像方法，我们发现培养物中贴壁细胞的质膜量在有丝分裂开始时减少，并在有丝分裂结束时迅速恢复。基于网格蛋白的内吞作用在细胞分裂的所有阶段都是正常的，而内化膜返回细胞表面的循环在四舍五入期间显着减慢，并在细胞膜开始恢复时恢复。通过遗传或化学手段干扰这些过程中的任何一个都会损害细胞分裂。即使没有功能性高尔基体，总细胞膜面积也会恢复，而高尔基体是输出新合成的膜脂和蛋白质所必需的。我们提出了一种机制，通过调节内体回收来控制细胞分裂过程中膜结合蛋白的细胞面积和表面表达。
The shape and total surface of a cell and its daughters change during mitosis. Many cells round up during prophase and metaphase and reacquire their extended and flattened shape during cytokinesis. How does the total area of plasma membrane change to accommodate these morphological changes and by what mechanism is control of total membrane area achieved? Using single-cell imaging methods, we have found that the amount of plasma membrane in attached cells in culture decreases at the beginning of mitosis and recovers rapidly by the end. Clathrin-based endocytosis is normal throughout all phases of cell division, whereas recycling of internalized membranes back to the cell surface slows considerably during the rounding up period and resumes at the time at which recovery of cell membrane begins. Interference with either one of these processes by genetic or chemical means impairs cell division. The total cell-membrane area recovers even in the absence of a functional Golgi apparatus, which would be needed for export of newly synthesized membrane lipids and proteins. We propose a mechanism by which modulation of endosomal recycling controls cell area and surface expression of membrane-bound proteins during cell division.