Cloning of the genome of a goose parvovirus vaccine strain SYG61v and rescue of infectious virions from recombinant plasmid in embryonated goose eggs
Cloning of the genome of a goose parvovirus vaccine strain SYG61v and rescue of infectious virions from recombinant plasmid in embryonated goose eggs
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鹅细小病毒疫苗株 SYG61v 基因组的克隆以及从鹅胚蛋中重组质粒中拯救感染性病毒粒子
DOI：
10.1016/j.jviromet.2014.02.014
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发表时间：
2014-05-01
影响因子：
3.1
通讯作者：
Zhu, Guoqiang
中科院分区：
文献类型：
--
作者：
Wang, Jianye;Duan, Jinkun;Zhu, Guoqiang
SYG61v是一种鹅细小病毒减毒株，已被中国用作疫苗株。对SYG761v的基因组进行了测序，试图确定该毒株减毒的遗传基础。全基因组由5102个核苷酸组成，与强毒B株相比有4个核苷酸缺失。反向末端重复序列(ITR)全长442个核苷酸，其中360个核苷酸形成一个茎区，43个核苷酸构成泡状区。虽然在整个ITR中观察到了突变，但在茎中没有发现错配。与其他GPV毒株(B、82-0321、06-0329和YZ99-5)的比对表明，SYG61v的Rep1和VP1蛋白分别有10个和11个氨基酸突变。将SYG61v全基因组克隆到pBluescrip II载体上，构建成感染性表达载体pSYG61v。将pSYG61v载体转入鹅胚蛋中，获得了与亲本病毒相似的病毒滴度，经ELD50检测，获得了感染后代病毒。(C)2014爱思唯尔B.V.保留所有权利。
The SYG61v is an attenuated goose parvovirus (GPV) that has been used as a vaccine strain in China. The genome of SYG761v was sequenced to attempt to identify the genetic basis for the attenuation of this strain. The entire genome consists of 5102 nucleotides (nts), with four nt deletions compared to that of virulent strain B. The inverted terminal repeats (ITR) are 442 nts in length, of which 360 nts form a stem region, and 43 nts constitute the bubble region. Although mutations were observed throughout the ITR, no mismatch was found in the stem. Alignment with other pathogenic.GPV strains (B, 82-0321,06-0329, and YZ99-5) indicated that there are 10 and 11 amino acid mutations in the Rep1 and VP1 proteins of SYG61v, respectively. The complete genome of SYG61v was cloned into the pBluescript II vector and an infectious plasmid pSYG61v was generated. Infectious progeny virus was successfully rescued through transfection of the plasmid pSYG61v in embryonated goose eggs and yielded viral titers similar to its parental virus, as evaluated by ELD50. (C) 2014 Elsevier B.V. All rights reserved.