Effects of a High-Fat Diet on Adipose Tissue CD8+T Cells in Young vs. Adult Mice
Effects of a High-Fat Diet on Adipose Tissue CD8+T Cells in Young vs. Adult Mice
复制标题
高脂肪饮食对幼年小鼠与成年小鼠脂肪组织 CD8 T 细胞的影响
DOI：
10.1007/s10753-017-0635-0
复制
发表时间：
2017-12-01
期刊：
影响因子：
5.1
通讯作者：
Wang, Hui
中科院分区：
文献类型：
--
作者：
Dong, Lijun;Zhang, Yao;Wang, Hui
T细胞参与肥胖状况下脂肪组织的慢性炎症。然而，年龄对脂肪T细胞的影响仍然未知。在这项研究中，我们调查了年轻和成年小鼠的白色脂肪组织中的T细胞。使用高脂饮食（HFD）在小鼠中诱导肥胖14周。幼鼠在3周龄时喂食HFD，成年鼠在12周龄时喂食HFD。与成年小鼠相比，年轻小鼠获得的脂肪更少，并且表现出更好的葡萄糖耐受性。他们的脂肪组织含有更多的CD8+ T细胞和更高水平的促炎细胞因子。年轻小鼠表现出更大的CD4+ T细胞增加。年轻和成年小鼠表现出相似的胰岛素耐受性。HFD降低结肠肌层，这在年轻小鼠中更明显。这些数据表明，年轻和成年小鼠表现出不同的反应HFD的脂肪组织，葡萄糖耐量，结肠肌肉层。CD8+ T细胞和CD4+ T细胞的增加，以及更高水平的促炎细胞因子，表明在年轻小鼠中脂肪增加较少的情况下炎症升高，这是出乎意料的。这种炎症的意义仍然未知。我们认为炎症可能会抑制脂肪组织中的能量储存，从而为年轻小鼠的瘦体重提供更多的能量，有利于其生长。本研究提供了炎症在生理条件下的有益作用的另一个例子。
T cells are involved in chronic inflammation of adipose tissue in obese conditions. However, the impact of age on the adipose T cells remains unknown. In this study, we investigated T cells in the white adipose tissue of young and adult mice. Obesity was induced in the mice using a high-fat diet (HFD) for 14 weeks. The young mice were fed an HFD at 3 weeks old, and adult mice were fed the HFD at 12 weeks old. Relative to adult mice, the young mice gained less fat and exhibited better glucose tolerance. Their adipose tissue contained more CD8+ T cells and higher levels of pro-inflammatory cytokines. Young mice showed a larger increase in CD4+ T cells. The young and adult mice showed similar insulin tolerance. HFD reduced the colon muscle layer, which was more obvious in the young mice. These data suggested that young and adult mice exhibit different responses to an HFD in terms of adipose tissue, glucose tolerance, and the colon muscle layer. The increase in CD8+ T cells and CD4+ T cells, together with higher levels of pro-inflammatory cytokines, suggested elevated inflammation in the presence of less fat gain in the young mice, which was unexpected. The significance of this inflammation remains unknown. We propose that inflammation might inhibit energy storage in the adipose tissue to provide more energy to the lean body mass in favor of growth in the young mice. The present study provides another example of the beneficial effect of inflammation in physiological conditions.