Egg laying suppression in Drosophila melanogaster (Diptera: Drosophilidae) and Dacus (Bactrocera) oleae (Diptera: Tephritidae) by phloroglucinol, a peroxidase inhibitor
Egg laying suppression in Drosophila melanogaster (Diptera: Drosophilidae) and Dacus (Bactrocera) oleae (Diptera: Tephritidae) by phloroglucinol, a peroxidase inhibitor
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DOI：
10.1017/s0007485300034957
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发表时间：
1996-08-01
影响因子：
1.9
通讯作者：
Tsiropoulos, GJ
中科院分区：
文献类型：
--
作者：
Keramaris, KE;Margaritis, LH;Tsiropoulos, GJ
卵壳过氧化物酶（ESP）在包括黑腹果蝇（Drosophila melanogaster Meigen）和橄榄实蝇（Bactrocera oleae Gmelin）在内的多种双翅目昆虫中负责硬化过程。在蛋壳形成过程中，它的作用可被间苯三酚（一种天然酚类物质）抑制。我们用间苯三酚，在饲料中的D。melanogaster和B.以1 mM、10 mM、25 mM、50 mM、100 mM和400 mM的浓度，将其加入到油橄榄中，以研究其对产蛋的影响。在这两种昆虫中，ImM没有影响。在10 mM以上浓度下，D.而B组则逐渐出现空壳和无绒毛膜卵沉积。oleae，它逐渐被抑制，只有无定形的群众奠定。间苯三酚在试验浓度下的作用是可逆的。结果表明，间苯三酚在适宜的非致死浓度下对杜父鱼的产卵有一定的影响。melanogaster和B. oleae在不同的方式，有关的差异，产卵器直径相对于鸡蛋的大小。在B。但是，由于绒毛膜蛋白质的共价交联被阻止，所以相对较窄，导致间苯三酚诱导的非弹性蛋壳破裂。In D.由于卵的脱绒作用，产下黑腹、无绒毛膜卵和单独的绒毛膜。这些结果的一个可能的现场应用进行了讨论。
Eggshell peroxidase (ESP) is responsible for the hardening process in several Diptera, including Drosophila melanogaster Meigen and Bactrocera oleae Gmelin. Its action can be inhibited by phloroglucinol, a natural phenolic substance, during the formation of the egg-shell. We used phloroglucinol, in the diet of adults of D. melanogaster and B. oleae, at concentrations of 1 mM, 10 mM, 25 mM, 50 mM, 100 mM and 400 mM to study its effect on egg laying. In both insects, 1 mM had no effect. At concentrations above 10 mM, egg laying of D. melanogaster was gradually affected leading to the deposition of empty shells and chorion-less eggs, while in B. oleae, it was gradually suppressed and only amorphous masses were laid. The effect of phloroglucinol at the tested concentrations was reversible. It is concluded that phloroglucinol added at appropriate, non-lethal concentrations, affected egg-laying of D. melanogaster and B. oleae in different ways, related to differences in the ovipositor diameter relative to egg size. In B. oleae, it is relatively narrow, causing breakage of the phloroglucinol-induced non-elastic egg-shell, since covalent crosslinking of the chorion proteins is prevented. In D. melanogaster, chorion-less eggs and separate chorions are laid, due to egg dechorionation. A possible field application of these results is discussed.