Assembly of regulatory factors on rRNA and ribosomal protein genes in Saccharomyces cerevisiae
Assembly of regulatory factors on rRNA and ribosomal protein genes in Saccharomyces cerevisiae
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DOI：
10.1128/mcb.00876-07
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发表时间：
2007-10-01
影响因子：
5.3
通讯作者：
Kokubo, Tetsuro
中科院分区：
文献类型：
--
作者：
Kasahara, Koji;Ohtsuki, Kazushige;Kokubo, Tetsuro
HMO 1是一种高迁移率B族蛋白，在酿酒酵母中编码rRNA和核糖体蛋白（RPGs）的基因转录中发挥作用。本研究使用全基因组染色质免疫沉淀来研究HMO 1、FHL 1和RAP 1在这些基因以及其他RNA聚合酶II转录的基因在酵母中的转录中的作用。结果表明，HMO 1与35 S rRNA基因在RNA聚合酶I依赖性的方式和RPG启动子（共138），可以分为几个不同的组的基础上，在启动子和HMO 1的FHL 1和/或RAP 1结合到启动子的HMO 1的依赖性HMO 1丰度。FHL 1是RPG的关键调节因子，以HMO 1依赖性方式与大多数HMO 1富集和转录HMO 1依赖性RPG启动子结合，而以HMO 1非依赖性方式与HMO 1限制性RPG启动子结合，无论它们是否以HMO 1依赖性方式转录。报告基因分析表明，这些功能特性是由启动子序列。
HMO1 is a high-mobility group B protein that plays a role in transcription of genes encoding rRNA and ribosomal proteins (RPGs) in Saccharomyces cerevisiae. This study uses genome-wide chromatin immunoprecipitation to study the roles of HMO1, FHL1, and RAP1 in transcription of these genes as well as other RNA polymerase II-transcribed genes in yeast. The results show that HMO1 associates with the 35S rRNA gene in an RNA polymerase I-dependent manner and that RPG promoters (138 in total) can be classified into several distinct groups based on HMO1 abundance at the promoter and the HMO1 dependence of FHL1 and/or RAP1 binding to the promoter. FHL1, a key regulator of RPGs, binds to most of the HMO1-enriched and transcriptionally HMO1-dependent RPG promoters in an HMO1-dependent manner, whereas it binds to HMO1-limited RPG promoters in an HMO1-independent manner, irrespective of whether they are transcribed in an HMO1-dependent manner. Reporter gene assays indicate that these functional properties are determined by the promoter sequence.