A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis
A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis
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从青蛙 Amolops loloensis 的皮肤分泌物中鉴定出一种新型库尼茨型神经毒素肽
DOI：
10.1016/j.bbrc.2020.05.051
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发表时间：
2020
影响因子：
3.1
通讯作者：
Liu Han
中科院分区：
文献类型：
--
作者：
Guo Xiaopu;Li Bowen;Liang Shaohui;Lai Ren;Liu Han
从棕点湍蛙（Amolops loloensis）皮肤分泌物中分离纯化了一种新的Kunitz型神经毒素肽，其cDNA全长240 bp，编码一个含6个半胱氨酸的79个氨基酸残基的前体蛋白。该前体被证明释放具有氨基酸序列DRNPICNLPPKEGFCLWMMRRSFNPSKGRCDTFGYRGCGGNKNNFETPRACKEACG的57-aa成熟肽。成熟的被命名为amotoxin。Amotoxin与其他Kunitz型毒素具有序列同源性，并且还具有三个半胱氨酸桥。Amotoxin对胰蛋白酶有抑制作用，抑制常数Ki为0.087 μM。据我们所知，这是第一个基因编码的神经毒素发现于Amolops loloensis。重组的amotoxin表现出与天然amotoxin相似的功能特性。这些功能特性有助于了解两栖动物的生态适应性，加深对两栖动物皮肤肽生物学功能谱的认识。
A novel Kunitz-type neurotoxin peptide that inhibited voltage-gated sodium channel was purified and characterized from the skin secretions of rufous-spotted torrent frog,Amolops loloensis.It has a 240-bp cDNA encoding an 79-amino acid residue (aa) precursor protein containing 6 half-cysteines. The precursor was proven to release a 57-aa mature peptide with amino acid sequence, DRNPICNLPPKEGFCLWMMRRSFFNPSKGRCDTFGYRGCGGNKNNFETPRACKEACG. The mature was named amotoxin. Amotoxin shares sequence homology with other Kunitz-type toxins and also has three cysteine bridges. Amotoxin showed an inhibitory ability against trypsin with an inhibitory constant (Ki) of 0.087 μM. To the best of our knowledge, this is the first gene-encoded neurotoxin found inAmolops loloensis. Recombinant amotoxin showed similar functional properties as the native amotoxin. The functional properties of amotoxin may provide insights into the ecological adaptation of amphibians and deepen our understanding about the biological function spectrum of amphibian skin peptides.