Comparison of neurolin (ALCAM) and neurolin-like cell adhesion molecule (NLCAM) expression in zebrafish
Comparison of neurolin (ALCAM) and neurolin-like cell adhesion molecule (NLCAM) expression in zebrafish
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DOI：
10.1016/j.modgep.2006.03.010
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发表时间：
2006-10-01
影响因子：
1.2
通讯作者：
Hughes, Simon M.
中科院分区：
文献类型：
--
作者：
Mann, Christopher J.;Hinits, Yaniv;Hughes, Simon M.
许多免疫球蛋白(Ig)超家族细胞黏附分子影响骨骼肌的形成。在果蝇中，目瞪口呆的(duf/kirre)、unC、棍子和石头以及Hibry编码在神经元和肌肉前体细胞亚群中表达的相关Ig家族蛋白。该家族介导细胞迁移、轴突引导和成肌细胞融合。尽管这些基因在无脊椎动物的肌肉发生中很重要，但在脊椎动物的肌肉发生中没有明显的功能相似之处。在这里，我们研究了DUF相关分子Neurlin和Neurlin样细胞黏附分子(NLCAM)的基因表达模式、系统发育和蛋白质结构关系，NLCAM是活化的白细胞黏附分子(ALCAM)亚家族的成员。这些蛋白质在序列上与Duf/Kirre最接近。在斑马鱼发育过程中，神经素在体节和肌肉细胞亚群、心脏和许多神经元成熟部位表达。新的alcam家族成员NLCAM似乎是通过复制Neurlin/alcam而产生的。NLCAM在原肠发育过程中广泛表达，特别是在新生的神经板中，但后来主要在肌肉和神经成熟部位以及鳍折中表达。每种基因的表达通常在胚胎相似部位的细胞群中；例如，在Rohon Beard神经元、三叉神经节和融合的快慢和迁移慢的肌肉纤维区域。然而，表达也可以是不同的和动态的；例如，肌肉前纤维高水平表达Neurlin，但不表达NLCAM。这两个分子在融合前的肌肉前体亚群中都有表达。(C)2006爱思唯尔B.V.保留所有权利。
Many immunoglobulin (Ig)-superfamily cell adhesion molecules influence skeletal muscle formation. In Drosophila, dumbfounded (duf/kirre), irreC, sticks and stones and hibris encode related Ig-family proteins expressed in subsets of neurons and muscle precursor cells. The family mediates cell migration, axon guidance and fusion of myoblasts. Despite the importance of these genes in invertebrate myogenesis, no obvious functional parallels are known in vertebrate myogenesis. Here we investigate the gene expression pattern and phylogenetic and protein-structural relationships of the duf-related molecules neurolin and neurolin-like cell adhesion molecule (NLCAM), members of the activated leukocyte cell adhesion molecule (ALCAM) sub-family of Ig-molecules. These proteins are among the closest to Duf/Kirre by sequence. During zebrafish development, neurolin is expressed in subsets of somite and muscle cells, heart and numerous sites of neuronal maturation. The new ALCAM-family member, NLCAM, appears to have arisen by duplication of neurolin/ALCAM. NLCAM is expressed widely during gastrulation, particularly in the nascent neural plate, but later becomes predominantly expressed in sites of muscle and nerve maturation and in the fin fold. The expression of each gene is often in groups of cells in similar parts of the embryo; for example, in the region of Rohon Beard neurons, trigeminal ganglion and fusing fast and migrating slow muscle fibres. However, expression can also be distinct and dynamic; for example, muscle pioneer fibres express neurolin but not NLCAM at high level. Both molecules are expressed in subsets of muscle precursors at times prior to fusion. (c) 2006 Elsevier B.V. All rights reserved.