A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.
A recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus.
复制标题
基于重组核衣壳蛋白的间接酶联免疫吸附测定法检测猪三角洲冠状病毒抗体
DOI：
10.1292/jvms.15-0533
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发表时间：
2016-05-03
期刊：
影响因子：
--
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Su M;Li C;Guo D;Wei S;Wang X;Geng Y;Yao S;Gao J;Wang E;Zhao X;Wang Z;Wang J;Wu R;Feng L;Sun D
最近，猪三角洲冠状病毒（PDCoV）已被证明与仔猪肠道疾病有关。与其他猪冠状病毒相比，PDCoV血清学调查的诊断工具仍处于开发阶段。在我们的研究中，间接酶联免疫吸附试验（ELISA）（rPDCoV-N-ELISA），以检测抗PDCoV抗体使用组氨酸标记的重组核衣壳（N）蛋白作为抗原。与猪流行性腹泻病毒、猪传染性胃肠炎病毒、猪A组轮状病毒、猪瘟病毒、猪圆环病毒2型、猪伪狂犬病病毒、猪繁殖与呼吸综合征病毒的抗血清无交叉反应;受试者工作特征（ROC）曲线分析显示，基于已知状态的样本，rPDCoV-N-ELISA的灵敏度为100%，特异性为90.4%（n= 10）。62）。使用rPDCoV-N-ELISA分析田间样品（n=319）表明，11.59%的样品对PDCoV抗体呈阳性。结果表明，rPDCoV-N-ELISA可用于PDCoV的流行病学调查，黑龙江省猪群中PDCoV的血清流行率较低。
Recently, porcine deltacoronavirus (PDCoV) has been proven to be associated with enteric disease in piglets. Diagnostic tools for serological surveys of PDCoV remain in the developmental stage when compared with those for other porcine coronaviruses. In our study, an indirect enzyme-linked immunosorbent assay (ELISA) (rPDCoV-N-ELISA) was developed to detect antibodies against PDCoV using a histidine-tagged recombinant nucleocapsid (N) protein as an antigen. The rPDCoV-N-ELISA did not cross-react with antisera against porcine epidemic diarrhea virus, swine transmissible gastroenteritis virus, porcine group A rotavirus, classical swine fever virus, porcine circovirus-2, porcine pseudorabies virus, and porcine reproductive and respiratory syndrome virus; the receiver operating characteristic (ROC) curve analysis revealed 100% sensitivity and 90.4% specificity of the rPDCoV-N-ELISA based on samples of known status (n=62). Analyses of field samples (n=319) using the rPDCoV-N-ELISA indicated that 11.59% of samples were positive for antibodies against PDCoV. These data demonstrated that the rPDCoV-N-ELISA can be used for epidemiological investigations of PDCoV and that PDCoV had a low serum prevalence in pig population in Heilongjiang province, northeast China.